Schmid Sonja, Dekker Cees
Nanodynamics Lab, Laboratory of Biophysics, Wageningen University, Stippeneng 4, 6708WE Wageningen, the Netherlands.
Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands.
iScience. 2021 Sep 25;24(10):103007. doi: 10.1016/j.isci.2021.103007. eCollection 2021 Oct 22.
This paper provides a perspective on potential applications of a new single-molecule technique, viz., the nanopore electro-osmotic trap (NEOtrap). This solid-state nanopore-based method uses locally induced electro-osmosis to form a hydrodynamic trap for single molecules. Ionic current recordings allow one to study an unlabeled protein or nanoparticle of arbitrary charge that can be held in the nanopore's most sensitive region for very long times. After motivating the need for improved single-molecule technologies, we sketch various possible technical extensions and combinations of the NEOtrap. We lay out diverse applications in biosensing, enzymology, protein folding, protein dynamics, fingerprinting of proteins, detecting post-translational modifications, and all that at the level of single proteins - illustrating the unique versatility and potential of the NEOtrap.
本文介绍了一种新型单分子技术——纳米孔电渗阱（NEOtrap）的潜在应用前景。这种基于固态纳米孔的方法利用局部诱导电渗作用形成一个用于捕获单分子的流体动力学阱。离子电流记录使人们能够研究任意电荷的未标记蛋白质或纳米颗粒，这些物质可以长时间被捕获在纳米孔最敏感的区域。在阐述了对改进单分子技术的需求之后，我们概述了NEOtrap的各种可能的技术扩展和组合方式。我们展示了其在生物传感、酶学、蛋白质折叠、蛋白质动力学、蛋白质指纹识别、检测翻译后修饰等方面的多种应用，所有这些都是在单蛋白质水平上进行的——这说明了NEOtrap独特的多功能性和潜力。
