Baran H, Jellinger K
Ludwig Boltzmann Institute of Clinical Neurobiology, Lainz Hospital, Vienna, Austria.
J Neural Transm Park Dis Dement Sect. 1992;4:267-76. doi: 10.1007/BF02260075.
Brain phenolsulfotransferase (PST) in 105.000 x g supernatant fractions prepared from post mortem human brain catalyzes the sulfate conjugation of dopamine (DA). Using 50 microM DA, the PST activity was linear up to one hour. The KM value for DA was 3.1 microM. Higher concentrations of DA from 25 b microM up caused inhibition of PST activity. Assessment of regional distribution in normal brain using 20 microM DA concentration revealed the highest PST activities in temporal and frontal cortex. About ten times lower activities were measured in parietal and occipital lobe, amygdala, hypothalamus, and hippocampus, whereas the nucleus accumbens, nucleus basalis of Meynert, caudate nucleus, and substantia nigra showed the lowest activities (about 1% of those in frontal and parietal cortex). In the brains of subjects with Parkinson's disease (PD) treated with levodopa, a significant reduction of PST activities was observed in hypothalamus, frontal and temporal cortex, amygdaloid nucleus, occipital and parietal cortex (between 20 and 38.8% of controls). Depletion of PST activity was less severe in hippocampus (46% of controls), nucleus accumbens, putamen, and substantia nigra (67 and 72% of controls, respectively). No changes were observed in the nucleus basalis of Meynert, while PST activity was increased in the caudate nucleus (174 to 203% of controls). The presented data indicate that on PD brain the PST activity is reduced in areas of the cerebral isocortex and limbic system, while in the basal ganglia it is either mildly reduced (putamen) or increased (caudate nucleus). Selective changes of PST activity in PD brain may indicate an important function of this enzyme in the metabolism and/or storage of DA under pathological conditions.
从人死后的大脑制备的105,000 x g上清液组分中的脑酚磺基转移酶（PST）催化多巴胺（DA）的硫酸化结合反应。使用50μM DA时，PST活性在长达一小时内呈线性。DA的KM值为3.1μM。25μM及以上更高浓度的DA会导致PST活性受到抑制。使用20μM DA浓度评估正常大脑中的区域分布，结果显示颞叶和额叶皮质的PST活性最高。在顶叶和枕叶、杏仁核、下丘脑和海马体中测得的活性约低十倍，而伏隔核、迈内特基底核、尾状核和黑质的活性最低（约为额叶和顶叶皮质活性的1%）。在用左旋多巴治疗的帕金森病（PD）患者的大脑中，下丘脑、额叶和颞叶皮质、杏仁核、枕叶和顶叶皮质的PST活性显著降低（为对照组的20%至38.8%）。海马体（为对照组的46%）、伏隔核、壳核和黑质（分别为对照组的67%和72%）的PST活性降低程度较轻。在迈内特基底核中未观察到变化，而尾状核中的PST活性增加（为对照组的174%至203%）。所呈现的数据表明，在PD大脑中，大脑新皮质和边缘系统区域的PST活性降低，而在基底神经节中，其活性要么轻度降低（壳核）要么增加（尾状核）。PD大脑中PST活性的选择性变化可能表明该酶在病理条件下DA的代谢和/或储存中具有重要作用。
