Barrera G, Martinotti S, Fazio V, Manzari V, Paradisi L, Parola M, Frati L, Dianzani M U
Toxicol Pathol. 1987;15(2):238-40. doi: 10.1177/019262338701500219.
The 4-hydroxynonenal aldehyde (HNE), a product of lipid peroxidation with high biological activity, inhibits cancerous growths in vivo and in vitro. The mechanism by which this aldehyde acts is not yet understood. The c-myc oncogene seems to be involved in the regulation of cellular multiplication and transformation. We evaluated the c-myc expression and the RNA, DNA and protein synthesis in K562 cells. These cells were incubated for 1 hour in presence of several aldehyde concentrations (range 5.10(-7) to 10(-4)), then washed and kept for 20 hours in a growth medium until used. HNE inhibited both the nucleic acids and protein synthesis in a dose dependent manner, and c-myc expression was evaluated in the K562 cells after incubation with 10(-4) M or 10(-6) M HNE. HNE inhibited c-myc expression only at the highest dose. These preliminary results may suggest that the inhibition of c-myc expression is related to nucleic acid synthesis inhibition following HNE exposure.
4-羟基壬烯醛（HNE）是脂质过氧化的产物，具有很高的生物活性，在体内和体外均能抑制癌细胞生长。这种醛发挥作用的机制尚不清楚。c-myc癌基因似乎参与细胞增殖和转化的调控。我们评估了K562细胞中c-myc的表达以及RNA、DNA和蛋白质的合成。将这些细胞在几种醛浓度（范围为5×10⁻⁷至10⁻⁴）下孵育1小时，然后洗涤，并在生长培养基中保存20小时直至使用。HNE以剂量依赖的方式抑制核酸和蛋白质合成，并在用10⁻⁴M或10⁻⁶M HNE孵育后评估K562细胞中c-myc的表达。HNE仅在最高剂量时抑制c-myc表达。这些初步结果可能表明，HNE暴露后c-myc表达的抑制与核酸合成的抑制有关。
