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神经纤毛蛋白-1通过脂多糖存在下的 TGF-β1/Smads 信号通路对 CD4+CD25+调节性 T 细胞稳定性的贡献。

The contribution of neuropilin-1 in the stability of CD4 CD25 regulatory T cells through the TGF-β1/Smads signaling pathway in the presence of lipopolysaccharides.

机构信息

Department of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin, PR China.

Department of Emergency Medicine, Rizhao People's Hospital of Shandong Province, Rizhao, PR China.

出版信息

Immun Inflamm Dis. 2022 Feb;10(2):143-154. doi: 10.1002/iid3.551. Epub 2021 Nov 10.

DOI:10.1002/iid3.551
PMID:34758202
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8767517/
Abstract

INTRODUCTION

This study investigates the synergistic effect of TGF-β1 and Nrp-1 on CD4 CD25 T ' stabilization, and the associated pathways of signal transduction, in vitro models in the presence of LPS.

MATERIALS AND METHODS

Spleen CD4 CD25 T cells of mice models in the presence of LPS, were transfected with an shRNA targeting Nrp-1, Smad2, or Smad3, may or may not be treated with recombinant TGF-β1. Followed by subsequent determination of cellular proliferation, rate of apoptosis, observation of the Foxp3, CTLA-4, and TGF-β1 expression levels, foxp3-TSDR methylation, secretion levels of the inhibitory cytokines IL-10 and TGF-β1, and Smad2/3 of CD4 CD25 T expression.

RESULTS

A remarkable stimulation in CD4 CD25 T ' stability is noted after administering recombinant TGF-β1 in the presence of LPS, and promoted cellular viability, increased Foxp3, CTLA-4, and TGF-β1 expression, and elevated secretion of IL-10 and TGF-β1. This also inhibited the apoptosis and methylation of foxp3- TSDR of CD4 CD25 T . The shRNA transfection silenced Nrp-1 and Smad3, but not Smad2, resulting in the suppression of the recombinant TGF-β1-mediated effects in the presence of LPS.

CONCLUSIONS

According to the results, Nrp-1 mediates TGF-β1 to improve the stability of regulatory CD4 CD25 T cells and maybe a possible therapeutic target with the ability to improve the CD4 CD25 T associated negative immunoregulation that is related to the TGF-β1/Smads cell signaling during sepsis.

摘要

简介

本研究旨在探讨 TGF-β1 和 Nrp-1 在 LPS 存在的体外模型中对 CD4 CD25 T'稳定性的协同作用，以及相关的信号转导途径。

材料和方法

在 LPS 存在的情况下，用靶向 Nrp-1、Smad2 或 Smad3 的 shRNA 转染小鼠模型的脾 CD4 CD25 T 细胞，然后用重组 TGF-β1 处理或不处理。随后测定细胞增殖率、凋亡率、Foxp3、CTLA-4 和 TGF-β1 表达水平、foxp3-TSDR 甲基化、抑制性细胞因子 IL-10 和 TGF-β1 的分泌水平以及 CD4 CD25 T 表达的 Smad2/3。

结果

在 LPS 存在的情况下给予重组 TGF-β1 可显著刺激 CD4 CD25 T'稳定性，并促进细胞活力、增加 Foxp3、CTLA-4 和 TGF-β1 表达以及 IL-10 和 TGF-β1 的分泌。这也抑制了 CD4 CD25 T 的凋亡和 foxp3-TSDR 的甲基化。shRNA 转染沉默了 Nrp-1 和 Smad3，但不沉默 Smad2，从而抑制了 LPS 存在下重组 TGF-β1 介导的作用。

结论

根据结果，Nrp-1 介导 TGF-β1 改善调节性 CD4 CD25 T 细胞的稳定性，可能是一种潜在的治疗靶点，能够改善与脓毒症期间 TGF-β1/Smads 细胞信号转导相关的 CD4 CD25 T 相关负免疫调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e304/8767517/d41a098777a4/IID3-10-143-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e304/8767517/979c656bb14d/IID3-10-143-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e304/8767517/7396479b0a49/IID3-10-143-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e304/8767517/c244975f8bcb/IID3-10-143-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e304/8767517/948943a7260b/IID3-10-143-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e304/8767517/d41a098777a4/IID3-10-143-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e304/8767517/979c656bb14d/IID3-10-143-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e304/8767517/7396479b0a49/IID3-10-143-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e304/8767517/c244975f8bcb/IID3-10-143-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e304/8767517/948943a7260b/IID3-10-143-g001.jpg
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3
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