Zhang Qiaoya, Yu Ying, Song Chunhui, Cao Zhi
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China; Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, China; Qingdao Research Center for Veterinary Biological Engineering and Technology, China.
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China; Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, China; Qingdao Research Center for Veterinary Biological Engineering and Technology, China.
Microb Pathog. 2021 Dec;161(Pt A):105282. doi: 10.1016/j.micpath.2021.105282. Epub 2021 Nov 7.
Atypical porcine pestivirus (APPV) is one major causative agent of congenital tremor (CT) type A-II. In this study, the geographical distribution and genetic characteristics of APPV strains in China were established. To date, APPV has been detected in at least ten provinces/regions of China. Genetic variability analysis showed that NS3 genes were highly conserved among Chinese APPV strains, while NS5A-, N-, and E-encoding genes were highly variable. Phylogenetic analysis revealed that all of the reference strains could be classified into 3 genotypes (1-3) and 7 subgenotypes (1.1-1.7). The Chinese APPV strains were assigned to all 3 genotypes and to 5 subgenotypes (1.2 and 1.4-1.7) of genotype 1. Amino acid insertions/deletions in nonstructural proteins, including NS3, NS5A, and NS5B, could only be found in Chinese APPV strains. Among the three envelope glycosylation proteins (E, E1, and E2), E was the most heavily glycosylated protein, and the N-glycosylation sites of E2 in different APPV strains showed apparent correlations with genetic types. Furthermore, a novel APPV strain named China/HeN01/2018 belonging to genotype 2 was identified in Henan Province. It was then isolated and successfully propagated in embryonic porcine kidney epithelial cells (SPEV cells). This study provides updated information to better understand the divergence of APPV strains in China. This first successful isolation of a Chinese APPV strain provides a research foundation for future studies.
非典型猪瘟病毒（APPV）是A型-II型先天性震颤（CT）的一种主要致病因子。在本研究中，确定了中国APPV毒株的地理分布和遗传特征。迄今为止，在中国至少十个省/地区检测到了APPV。遗传变异性分析表明，中国APPV毒株的NS3基因高度保守，而编码NS5A、N和E的基因高度可变。系统发育分析显示，所有参考毒株可分为3个基因型（1-3）和7个亚基因型（1.1-1.7）。中国的APPV毒株被归入所有3个基因型以及基因型1的5个亚基因型（1.2和1.4-1.7）。非结构蛋白（包括NS3、NS5A和NS5B）中的氨基酸插入/缺失仅在中国APPV毒株中发现。在三种包膜糖基化蛋白（E、E1和E2）中，E是糖基化程度最高的蛋白，不同APPV毒株中E2的N-糖基化位点与遗传类型表现出明显的相关性。此外，在河南省鉴定出一株属于基因型2的新型APPV毒株，命名为China/HeN01/2018。然后将其分离并在猪胚胎肾上皮细胞（SPEV细胞）中成功传代。本研究提供了最新信息，以更好地了解中国APPV毒株的差异。中国APPV毒株的首次成功分离为未来的研究提供了基础。
