Saputri Fadlina Chany, Hutahaean Irma, Mun'im Abdul
Department of Pharmacology-Toxicology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok 16424, West Java, Indonesia.
Graduate Program of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok 16424, West Java, Indonesia.
Saudi J Biol Sci. 2021 Nov;28(11):6191-6197. doi: 10.1016/j.sjbs.2021.06.075. Epub 2021 Jun 30.
(L.) Kunth has been used widely to treat headache, kidney disease, fever, and hypertension. Previous studies discovered that the flavonoid-rich extract of this plant has potential hypotensive effects, specifically angiotensin-converting enzyme (ACE)-inhibitory activity. However, there is insufficient scientific evidence to validate the result .
This study investigated the dose dependencies of the effects of the ethyl acetate fraction of the ethanolic extract of this plant on blood pressure and biomarkers associated with the renin-angiotensin-aldosterone systems (RAAS), such as angiotensin II (AII) and the plasma renin concentration (PRC).
In total, 30 two-kidney, one-clip (2K1C) hypertensive model rats were divided into five groups (n = 6 each): model group, captopril 25 mg/kg BW group, and three different ethyl acetate groups (25, 50, and 100 mg/kg BW). Another six rats comprised the sham group.
Renal hypertensive rats (RHRs) were generated using stainless steel modification clips. Drugs were administered via oral gavage for 2 consecutive weeks. Blood pressure was measured weekly prior to treatment. Blood samples were collected before treatment and after the last dose to measure AII and PRC. The left kidney was isolated for histopathological examination.
Blood pressure, AII levels, and PRC were elevated after 6 weeks in RHRs. Treatment with captopril and the ethyl acetate fraction of (L.) Kunth decreased blood pressure, AII levels, and PRC. The ethyl acetate fraction at a dose of 50 mg/kg BW had similar ACE-inhibitory effects as captopril. Histopathological examination disclosed coagulative necrosis in clipped kidneys. Impairment was alleviated in a dose-dependent manner by (L.) Kunth, similarly as observed in the captopril group.
(L.) Kunth targets the renin-angiotensin-aldosterone system, which might explain its antihypertensive effects.
昆特草（L.）Kunth已被广泛用于治疗头痛、肾脏疾病、发热和高血压。先前的研究发现，这种植物富含黄酮类化合物的提取物具有潜在的降压作用，特别是血管紧张素转换酶（ACE）抑制活性。然而，尚无足够的科学证据来证实这一结果。
本研究调查了该植物乙醇提取物乙酸乙酯部分对血压以及与肾素-血管紧张素-醛固酮系统（RAAS）相关的生物标志物（如血管紧张素II（AII）和血浆肾素浓度（PRC））影响的剂量依赖性。
总共30只两肾一夹（2K1C）高血压模型大鼠分为五组（每组n = 6）：模型组、卡托普利25 mg/kg体重组和三个不同的乙酸乙酯组（25、50和100 mg/kg体重）。另外六只大鼠组成假手术组。
使用不锈钢改良夹制作肾性高血压大鼠（RHRs）。连续2周经口灌胃给药。在治疗前每周测量血压。在治疗前和最后一剂后采集血样以测量AII和PRC。分离左肾进行组织病理学检查。
RHRs在6周后血压、AII水平和PRC升高。用卡托普利和昆特草（L.）Kunth的乙酸乙酯部分治疗可降低血压、AII水平和PRC。50 mg/kg体重剂量的乙酸乙酯部分具有与卡托普利相似的ACE抑制作用。组织病理学检查显示夹闭肾脏出现凝固性坏死。昆特草（L.）Kunth以剂量依赖性方式减轻损伤，与卡托普利组观察到的情况相似。
昆特草（L.）Kunth作用于肾素-血管紧张素-醛固酮系统，这可能解释了其降压作用。
