Cardioprotective Effect of against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac Biomarkers.

OBJECTIVES

This study assessed the electrocardiographic pattern and cardiac inflammatory response of doxorubicin-induced myocardial injury in Wistar rats treated with ethanol extract.

METHODS

Female Wistar rats (190-200 g) were assigned into five groups of seven rats each. The Group 1 (Control group) was given rat chow and drinking water while the Group 2 (doxorubicin group) received intraperitoneal administration of doxorubicin (2 mg/kg) once weekly for three weeks. The Group 3 ( group) received 200 mg/kg of ethanolic extract of daily. Group 4 (Doxorubicin + group) received doxorubicin in addition to Group 5 (Captopril (50 mg/kg) was administered to another group in addition to while the doxorubicin + captopril group was administered captopril in addition to doxorubicin. Electrical recording and cardiac markers were evaluated.

RESULTS

The results revealed a significant (p < 0.01) elevation of T-wave and altered electrocardiographic parameters in the doxorubicin group than the control, , and other experimental groups. The heart rate, cardiac troponin level, lactate dehydrogenase, creatine kinase, angiotensin-converting enzyme activities, and inflammatory biomarkers were significantly (p < 0.01) higher while nitric oxide level was significantly (p < 0.05) reduced in the doxorubicin-only group compared to the control. Cardiac cell hypertrophy and inflammatory cell infiltration were observed due to doxorubicin administration. Treatment with extract and captopril reversed these trends and improved the antioxidants and inflammatory activities.

CONCLUSION

extract improves electrocardiographic pattern, has cardioprotective ability, and prevents doxorubicin-induced myocardial injury probably due to its phytochemical constituents and anti-inflammatory properties.