Madaiah Hemalata, Tarannum Fouzia, Faizuddin Mohamed
Department of Periodontics, M. R. Ambedkar Dental College and Hospital, Bengaluru, Karnataka, India.
Dent Res J (Isfahan). 2021 Sep 25;18:79. doi: 10.4103/1735-3327.326652. eCollection 2021.
Adipocyte fatty acid binding protein (A-FABP) is a novel biomarker of inflammation for various chronic systemic diseases. Since periodontitis is a chronic inflammatory disease, this study explores the association of A-FABP with periodontal disease parameters and tumor necrosis factor-alpha (TNF-α) levels in gingival crevicular fluid in periodontal health and disease.
This original research article describes a cross-sectional study conducted at the Department of Periodontics, M. R. Ambedkar Dental College and Hospital, Bangalore, India. This cross-sectional investigation was conducted on sixty subjects which were divided into three groups of twenty subjects each - healthy, gingivitis, and chronic periodontitis. Clinical parameters - plaque index, bleeding index, probing depth, and clinical attachment loss were recorded. Gingival crevicular fluid samples were analyzed for A-FABP and TNF-α levels using ELISA. One-way analysis of variance was used to find the significance of study parameters on a continuous scale between three groups. Pearson's correlation has been used to find the relationship between Gingival crevicular fluid concentration of markers and periodontal parameters. Multiple linear regression analysis was applied to the study. The statistical significance was considered at < 0.05.
Mean concentration of A-FABP (6.43 ± 2.51) and TNF-α (3454.82 ± 1566.44) was highest in the periodontitis group, and the difference among the groups was statistically significant ( < 0.05). A positive correlation was observed between clinical attachment loss and the two markers among all groups. The correlation between A-FABP and TNF-α in periodontitis groups was positive and statistically significant ( < 0.05). Multiple linear regression model was statistically significant ( < 0.05) indicating that there is a significant relationship between the set of predictors and the clinical attachment loss.
A-FABP and TNF-α levels in GCF were significantly elevated in the presence of inflammation. A-FABP has a probable stimulatory effect on TNF-α; however, its role needs to be explored. A-FABP could serve as a novel inflammatory biomarker of periodontitis and the scope of using A-FABP inhibition as a treatment modality could be investigated with interventional studies.
脂肪细胞脂肪酸结合蛋白（A-FABP）是多种慢性全身性疾病的一种新型炎症生物标志物。由于牙周炎是一种慢性炎症性疾病，本研究探讨了牙周健康和疾病状态下A-FABP与牙周疾病参数以及龈沟液中肿瘤坏死因子-α（TNF-α）水平之间的关联。
这篇原创研究文章描述了在印度班加罗尔M.R.安贝德卡尔牙科学院及医院牙周病科进行的一项横断面研究。该横断面调查针对60名受试者展开，他们被分为三组，每组20名受试者，分别为健康组、牙龈炎组和慢性牙周炎组。记录了临床参数——菌斑指数、出血指数、探诊深度和临床附着丧失情况。使用酶联免疫吸附测定法（ELISA）分析龈沟液样本中的A-FABP和TNF-α水平。采用单因素方差分析来确定三组之间连续尺度上研究参数的显著性。使用皮尔逊相关性分析来确定标志物的龈沟液浓度与牙周参数之间的关系。对该研究应用了多元线性回归分析。统计学显著性设定为<0.05。
牙周炎组中A-FABP的平均浓度（6.43±2.51）和TNF-α的平均浓度（3454.82±1566.44）最高，且组间差异具有统计学显著性（<0.05）。在所有组中，临床附着丧失与这两种标志物之间均观察到正相关性。牙周炎组中A-FABP与TNF-α之间的相关性为正且具有统计学显著性（<0.05）。多元线性回归模型具有统计学显著性（<0.05），表明预测变量集与临床附着丧失之间存在显著关系。
在存在炎症的情况下，龈沟液中A-FABP和TNF-α水平显著升高。A-FABP可能对TNF-α有刺激作用；然而，其作用仍有待探索。A-FABP可作为牙周炎的一种新型炎症生物标志物，并且可以通过干预性研究来探讨使用A-FABP抑制作为一种治疗方式的可能性。
