Reisie Bentol Hoda, Farhad Shirin Zahra, Sadeh Shirin Amini
Department of Periodontics, Faculty of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
Dent Res J (Isfahan). 2021 Oct 21;18:82. eCollection 2021.
The tissue engineering has recently shown a significant progress in the fields of membranes and biosynthetic materials. Advanced platelet-rich fibrin (A-PRF) contains functional molecules that have newly shown great interest in regenerative therapies. The purpose of this study was to evaluate the effect of A-PRF on the adhesion of gingival fibroblast cells and osteosarcoma cells to different membranes.
In this experimental study, three collagen, alloderm, and mucograft membranes were studied, which were cut into four 5 mm × 5 mm pieces and placed in the bottom of a 24-well culture medium. One milliliter of A-PRF was added to two wells from each group and the other two wells remained without A-PRF. The gingival fibroblasts and osteosarcoma cells were individually added to each well. The cell adhesion was studied using an electron microscope after 24 h. The data were analyzed by independent -test, one-way analysis of variance, and least significant difference test.
In the presence of A-PRF, there was a significant higher osteoblast adhesion to collagen membrane compared to alloderm and mucograft membranes ( < 0.001). In the absence of A-PRF, adhesion of osteoblasts to collagen membrane was significantly higher than alloderm and mucograft ( = 0.019). Moreover, in the presence of A-PRF, fibroblast adhesion to collagen membrane was significantly higher than alloderm and mucograft membranes ( < 0.001). Furthermore, in the absence of A-PRF, no significant difference was found among the study groups ( = 0.830).
A-PRF was effective on fibroblast adhesion to the collagen membrane, which is similar to its absence. A-PRF was also found to be very effective on the adhesion of fibroblast cells to the collagen membrane, and in its absence, even less adhesion was observed compared to the other membranes. The presence or absence of A-PRF showed no significant differences in both cells' adhesion for alloderm and mucograft membranes.
组织工程学最近在膜和生物合成材料领域取得了显著进展。高级富血小板纤维蛋白（A-PRF）含有功能性分子，这些分子最近在再生疗法中引起了极大的关注。本研究的目的是评估A-PRF对牙龈成纤维细胞和骨肉瘤细胞黏附于不同膜的影响。
在本实验研究中，对三种胶原膜、同种异体真皮和黏膜移植膜进行了研究，将其切成四个5mm×5mm的小块，并放置在24孔培养基的底部。每组中两个孔加入1毫升A-PRF，另外两个孔不添加A-PRF。将牙龈成纤维细胞和骨肉瘤细胞分别加入每个孔中。24小时后使用电子显微镜研究细胞黏附情况。数据采用独立样本t检验、单因素方差分析和最小显著差异检验进行分析。
在有A-PRF存在的情况下，与同种异体真皮和黏膜移植膜相比，成骨细胞对胶原膜的黏附显著更高（P<0.001）。在没有A-PRF的情况下，成骨细胞对胶原膜的黏附显著高于同种异体真皮和黏膜移植膜（P = 0.019）。此外，在有A-PRF存在的情况下，成纤维细胞对胶原膜的黏附显著高于同种异体真皮和黏膜移植膜（P<0.001）。此外，在没有A-PRF的情况下，各研究组之间未发现显著差异（P = 0.830）。
A-PRF对成纤维细胞黏附于胶原膜有效，有无A-PRF情况类似。还发现A-PRF对成纤维细胞黏附于胶原膜非常有效，在没有A-PRF时，与其他膜相比黏附甚至更少。A-PRF的有无在两种细胞对同种异体真皮和黏膜移植膜的黏附方面均未显示出显著差异。
