Barekatain Mehrdad, Mafi Morvarid, Amini Shirin, Farhad Shirin Zahra
Department of Restorative Dentistry, Khorasgan Islamic Azad Dental School, Ahvaz, Iran.
Department of Periodontics, Jundishapur University of Medical Sciences, Ahvaz, Iran.
Dent Res J (Isfahan). 2014 Jul;11(4):429-35.
Tissue engineering represents very exciting advances in regenerative medicine; however, periodontal literature only contains few reports. Emdogain (EMD) consists of functional molecules that have shown many advantages in regenerative treatments. This study investigated EMD effect on gingival fibroblast adhesion to different membranes.
Two dense polytetrafluoroethylene membranes (GBR-200, TXT-200), Alloderm and a collagenous membrane (RTM Collagen) were used in this experimental study. Each membrane was cut into four pieces and placed at the bottom of a well in a 48-well plate. 10 μg/mL of EMD was added to two wells of each group. Two wells were left EMD free. Gingival fibroblasts were seeded to all the wells. Cell adhesion was evaluated by means of a Field Emission Scanning Electron Microscope after 24 hours incubation. Data was analyzed by independent t-test, one-way and two-way ANOVA and post hoc LSD test. P < 0.05 in independent t-test analysis and P < 0.001 in one-way ANOVA, two-way ANOVA and post hoc LSD analysis was considered statistically significant.
Alloderm had the highest cell adhesion capacity in EMD+ group and the difference was statistically significant (P < 0.001). In EMD- group, cell adhesion to TXT-200 and Alloderm was significantly higher than GBR-200 and collagenous membrane (P < 0.001).
This study showed that EMD may decrease the cell adhesion efficacy of GBR-200, TXT-200 and collagenous membrane but it can promote this efficacy in Alloderm. It also showed the composition of biomaterials, their surface textures and internal structures can play an important role in their cell adhesion efficacy.
组织工程代表了再生医学领域非常令人兴奋的进展；然而，牙周病学文献中仅有少数相关报道。Emdogain（EMD）由多种功能分子组成，这些分子在再生治疗中已显示出诸多优势。本研究调查了EMD对牙龈成纤维细胞黏附于不同膜材料的影响。
本实验研究使用了两种致密聚四氟乙烯膜（GBR - 200、TXT - 200）、同种异体脱细胞真皮基质（Alloderm）和一种胶原膜（RTM Collagen）。将每种膜切成四块，置于48孔板的孔底部。每组的两个孔中添加10μg/mL的EMD。另外两个孔不添加EMD。将牙龈成纤维细胞接种到所有孔中。孵育24小时后，通过场发射扫描电子显微镜评估细胞黏附情况。数据采用独立样本t检验、单因素方差分析、双因素方差分析以及事后LSD检验进行分析。独立样本t检验分析中P < 0.05，单因素方差分析、双因素方差分析以及事后LSD分析中P < 0.001被认为具有统计学意义。
在EMD +组中，同种异体脱细胞真皮基质的细胞黏附能力最强，差异具有统计学意义（P < 0.001）。在EMD -组中，细胞对TXT - 200和同种异体脱细胞真皮基质的黏附显著高于GBR - 200和胶原膜（P < 0.001）。
本研究表明，EMD可能会降低GBR - 200、TXT - 200和胶原膜的细胞黏附效果，但可提高同种异体脱细胞真皮基质的细胞黏附效果。研究还表明，生物材料的组成、表面纹理和内部结构在其细胞黏附效果中可发挥重要作用。
