Rodrigues Annelissa Zorzeto, Oliveira Paulo Tambasco de, Novaes Arthur Belém, Maia Luciana Prado, Souza Sérgio Luís Scombatti de, Palioto Daniela Bazan
Department of Oral and Maxillofacial Surgery and Traumatology and Periodontology, University of São Paulo, Ribeirão Preto, SP, Brazil.
Braz Dent J. 2010;21(3):179-89. doi: 10.1590/s0103-64402010000300001.
The acellular dermal matrix (ADM) was introduced in periodontology as a substitute for the autogenous grafts, which became restricted because of the limited source of donor's tissue. The aim of this study was to investigate, in vitro, the distribution, proliferation and viability of human gingival fibroblasts seeded onto ADM. ADM was seeded with human gingival fibroblasts for up to 21 days. The following parameters were evaluated: cell distribution, proliferation and viability. Results revealed that, at day 7, fibroblasts were adherent and spread on ADM surface, and were unevenly distributed, forming a discontinuous single cell layer; at day 14, a confluent fibroblastic monolayer lining ADM surface was noticed. At day 21, the cell monolayer exhibited a reduction in cell density. At 7 days, about to 90% of adherent cells on ADM surface were cycling while at 14 and 21 days this proportion was significantly reduced. A high proportion of viable cell was detected on AMD surface both on 14 and 21 days. The results suggest that fibroblast seeding onto ADM for 14 days can allow good conditions for cell adhesion and spreading on the matrix; however, migration inside the matrix was limited.
脱细胞真皮基质（ADM）作为自体移植物的替代品被引入牙周病学领域，由于供体组织来源有限，自体移植物的应用受到了限制。本研究的目的是在体外研究接种于人牙龈成纤维细胞的ADM上细胞的分布、增殖和活力。将人牙龈成纤维细胞接种到ADM上培养长达21天。评估以下参数：细胞分布、增殖和活力。结果显示，在第7天，成纤维细胞附着并铺展在ADM表面，分布不均匀，形成不连续的单细胞层；在第14天，观察到ADM表面有一层融合的成纤维细胞单层。在第21天，细胞单层的细胞密度降低。在第7天，ADM表面约90%的贴壁细胞处于增殖状态，而在第14天和第21天，这一比例显著降低。在第14天和第21天，ADM表面均检测到高比例的活细胞。结果表明，将成纤维细胞接种到ADM上14天可为细胞在基质上的黏附和铺展创造良好条件；然而，细胞在基质内的迁移受到限制。
