Dehghani Nazhvani Ali, Hosseini Seyed-Mojtaba, Tahoori Bita, Tavangar Maryam-Sadat, Attar Armin
Biomaterial Research Center, Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Cell and Molecular Research Club, Shiraz University of Medical Sciences, Shiraz, Iran.
J Dent (Shiraz). 2015 Sep;16(3 Suppl):246-50.
Stem cells are considered as new implement for tissue regeneration. Several niches in adult human body are colonized by multipotent stem cells but access to these potential reservoirs is often limited. Although human dental pulp stem cells isolated from healthy teeth have been extensively characterized, it is still unknown whether stem cells also exist in reactive lesions of oral cavity such as pyogenic granuloma and peripheral ossifying fibroma which are deliberated as inflammatory proliferation of different cell families.
The aim of this study was to explore for clues to see whether pyogenic granuloma or peripheral ossifying fibroma contain dental mesenchymal stem cell (DMSC).
Four pyogenic granuloma and four peripheral ossifying fibroma specimens were collected by excisional biopsy and preserved in PBS-EDTA at -86 °C. Then we cut them in 5µm diameter using Cryostat. Having been rinsed with PBS, the samples were stained with a primary mouse anti-human STRO-1 monoclonal IgM antibody. Afterward, a secondary goat anti-mouse IgM-FITC antibody was applied to detect STRO-1+ cells as probable stem cells by immunofluorescence technique.
Immunofluorescence microscopy revealed presence of STRO-1+ cells in these lesions, particularly localized on perivascular zone. The negative control group was not glowing.
Based on these results, it was found that reactive lesions of pyogenic granuloma and peripheral ossifying fibroma have STRO-1 positive cells, which raises the possibility that these cells may be DMSCs.
干细胞被视为组织再生的新工具。成人体内的几个生态位被多能干细胞占据，但获取这些潜在储存库的途径往往有限。尽管从健康牙齿中分离出的人牙髓干细胞已得到广泛表征，但口腔反应性病变如化脓性肉芽肿和外周骨化性纤维瘤（被认为是不同细胞家族的炎症性增殖）中是否也存在干细胞仍不清楚。
本研究的目的是探索化脓性肉芽肿或外周骨化性纤维瘤是否含有牙间充质干细胞（DMSC）的线索。
通过切除活检收集4个化脓性肉芽肿和4个外周骨化性纤维瘤标本，并保存在-86°C的PBS-EDTA中。然后用低温恒温器将它们切成5μm直径。用PBS冲洗后，样品用小鼠抗人STRO-1单克隆IgM抗体进行染色。随后，应用山羊抗小鼠IgM-FITC二抗通过免疫荧光技术检测作为可能干细胞的STRO-1+细胞。
免疫荧光显微镜显示这些病变中存在STRO-1+细胞，特别是位于血管周围区域。阴性对照组未发光。
基于这些结果，发现化脓性肉芽肿和外周骨化性纤维瘤的反应性病变中有STRO-1阳性细胞，这增加了这些细胞可能是DMSC的可能性。
