Effects of total mixed ration with various silage on growth performance, serum parameters, ruminal fermentation, and bacteria community profile in beef cattle.

The possibilities of using high-quality forages in incorporation with total mixed ration (TMR) during the fattening period of beef cattle have been investigated. A total of 30 Simmental bulls (438.94 ± 11.45 kg) were selected and randomly divided into two groups, TMR with single corn silage (SS) and TMR with various silage (MS). The whole experiment consisted of 15 days preparation period and 69 days experimental period. Rumen fluid and blood samples were taken from six beef cattle per treatment at the end of the experiment. The results showed that the average daily gain of the MS group (1.56 kg/day) was higher than ( < .05) the SS group (1.30 kg/day), and a decrease of feed conversion ratio in the MS (10.83) group was observed compared with SS group (12.36) ( < .05). The concentration of total volatile fatty acids for MS group was greater than ( < .05) the SS group. The activities of total antioxidant capacity and superoxide dismutase from MS group were also higher than the SS group, but lower urea nitrogen was found in the MS group from serum ( < .05). In addition, the abundances of the and were higher in the MS group than the SS group ( < .05). An increase in the flavonoid biosynthesis was detected in the MS group compared with the SS group by Kyoto Encyclopaedia of Genes and Genomes analysis. The present findings suggest that it is economical and healthy to substitute high-quality forage +low level of concentrate for a relatively low proportion forage +high level of concentrate in a finishing diet of beef cattle, which was a feasible and healthy strategy in the intensive feeding system.