Xie Lan, Xing Jingya, Qi Xingzhen, Lu Ting, Jin Yaqian, Akhtar Muhammad Faheem, Li Lanjie, Liu Guiqin
Shandong Engineering Technology Research Center for Efficient Breeding and Ecological Feeding of Black Donkey, Shandong Donkey Industry Technology Collaborative Innovation Center, College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng 252000, China.
College of Animal Science, Qingdao Agricultural University, Qingdao 266000, China.
Animals (Basel). 2023 Sep 12;13(18):2893. doi: 10.3390/ani13182893.
In this study, effects on the growth performance, nutrient digestibility, volatile fatty acids (VFA) production, and fecal microbiota of weaned donkeys were observed using different concentrate feeding sequences. Fifteen healthy 6-month-old weaned male donkeys with a body weight of 117.13 ± 10.60 kg were randomly divided into three treatment groups, including group C1 (roughage-then-concentrate), group C2 (concentrate-then-roughage), and group C3 (total mixed ration, TMR). The experiment lasted 35 d. We measured nutrient digestion by the acid-insoluble ash method and analyzed the fecal microbiota of the weaned donkeys by high-throughput sequencing of 16s rRNA genes in the V-V region. The results show that group C3 obtained the best growth performance, and the digestibility of crude protein (CP) and crude extract (EE) was significantly higher than that of group C1 ( < 0.05). Acetic acid, isobutyric acid, valeric acid, isovaleric acid, and caproic acid were notably different among all groups ( < 0.05). In addition, we observed that Firmicutes and Bacteroidetes were dominant in the fecal microbes of each group, and Firmicutes was significantly higher in group C3 ( < 0.05). At the genus level, the different genera were Treponema, Rikenellaceae-RC9-gut-group, Unidentified-F082, and Bacteroidales-RF16-group ( < 0.05). The prediction of fecal microbiota function by PICRUSt indicated that different feeding sequences had minimal impact on the function of the fecal microbiota, particularly on the high-abundance pathway. In summary, the concentrate feeding sequence changed the composition of the fecal microbe of weaned donkeys.
在本研究中，通过不同的精料饲喂顺序，观察其对断奶驴生长性能、养分消化率、挥发性脂肪酸（VFA）产生及粪便微生物群的影响。选取15头体重为117.13±10.60 kg的6月龄健康断奶雄性驴，随机分为三个处理组，即C1组（粗饲料后精料）、C2组（精料后粗饲料）和C3组（全混合日粮，TMR）。试验持续35天。采用酸不溶性灰分法测定养分消化率，并通过对V-V区16s rRNA基因进行高通量测序分析断奶驴的粪便微生物群。结果表明，C3组生长性能最佳，粗蛋白（CP）和粗脂肪（EE）消化率显著高于C1组（P<0.05）。各处理组间乙酸、异丁酸、戊酸、异戊酸和己酸差异显著（P<0.05）。此外，观察到厚壁菌门和拟杆菌门在各组粪便微生物中占主导地位，C3组厚壁菌门显著更高（P<0.05）。在属水平上，不同的属为密螺旋体属、理研菌科-RC9肠道群、未鉴定-F082和拟杆菌目-RF16群（P<0.05）。通过PICRUSt对粪便微生物群功能进行预测表明，不同的饲喂顺序对粪便微生物群功能影响极小，尤其是对高丰度通路。综上所述，精料饲喂顺序改变了断奶驴粪便微生物的组成。
