Huang Shasha, Zhang Haicheng, Qin Xiaopeng, Nie Chengzhen, Yu Xiao, Deng Qianchun
College of Food and Bioengineering Zhengzhou University of Light Industry Henan Key Laboratory of Cold Chain Food Quality and Safety Control, and Collaborative Innovation Center for Food Production and Safety Zhengzhou China.
Oil Crops Research Institute Chinese Academy of Agricultural Sciences Hubei Key Laboratory of Lipid Chemistry and Nutrition, and Key Laboratory of Oilseeds Processing, Ministry of Agriculture Wuhan China.
Food Sci Nutr. 2021 Sep 10;9(11):6031-6046. doi: 10.1002/fsn3.2538. eCollection 2021 Nov.
The endogenous fortification of antioxidant lipid concomitants in flaxseed was imperative to improve the oxidative stability of α-linolenic acid (ALA) in flaxseed and flaxseed oil upon processing, storage and gastrointestinal digestion. The comparative effects of acidic electrolyzed water (ACEW) and tap water (TW) on the triglyceride configuration, typical lipid concomitants, and antioxidant properties of flaxseed were conducted during 0-5 days of germination. The results showed that ACEW enhanced the germination rate of flaxseed by 18.25% and simultaneously suppressed the dynamic depletion of ALA by 5.32% when compared with TW ( < .05). The total phenolic acids, lignans, and flavonoids were effectively accumulated in flaxseed following ACEW-mediated germination with the further increase by 4.82%, 15.48%, and 8.22% in comparison with those induced by TW ( < .05). The total contents of cyclolinopeptides in flaxseed progressively dropped following either ACEW or TW treatment, a slighter decrease by 5.59% for flaxseed treated by ACEW than that by TW. Notably, the maximum accumulation of tocopherols and phytosterols had been early obtained for flaxseed treated with ACEW for 2-3 days due to the de novo synthesis or intermolecular conformational transition ( < .05). Most importantly, ACEW-mediated germination led to higher increment of the thermal oxidative stability and antioxidant properties of flaxseed and flaxseed oil in comparison to TW. In brief, the initial oxidation temperature increased by 7.09% and 3.06% ( < .05), and the antioxidant activities as evaluated by DPPH, ABTS, and FRAP values raised by 3.86%-28.07% and 4.21%-9.18% ( < .05), respectively. These findings clarify that the germination especailly mediated by ACEW could be an effective method to further optimize the nutritional and functional properties of flaxseed through reconstructing the endogenous antioxidant system.
亚麻籽中抗氧化脂质伴随物的内源性强化对于提高亚麻籽及亚麻籽油在加工、储存和胃肠道消化过程中α-亚麻酸(ALA)的氧化稳定性至关重要。在发芽的0-5天期间,研究了酸性电解水(ACEW)和自来水(TW)对亚麻籽甘油三酯构型、典型脂质伴随物和抗氧化性能的比较影响。结果表明,与TW相比,ACEW使亚麻籽的发芽率提高了18.25%,同时抑制了ALA的动态消耗达5.32%(P<0.05)。ACEW介导发芽后,亚麻籽中总酚酸、木脂素和黄酮类物质有效积累,与TW诱导的相比,分别进一步增加了4.82%、15.48%和8.22%(P<0.05)。ACEW或TW处理后,亚麻籽中环环肽的总含量逐渐下降,ACEW处理的亚麻籽比TW处理的下降幅度小5.59%。值得注意的是,由于从头合成或分子间构象转变(P<0.05),ACEW处理2-3天的亚麻籽早期获得了生育酚和植物甾醇的最大积累量。最重要的是,与TW相比,ACEW介导的发芽导致亚麻籽和亚麻籽油的热氧化稳定性和抗氧化性能有更高的提升。简而言之,初始氧化温度分别提高了7.09%和3.06%(P<0.05),通过DPPH、ABTS和FRAP值评估的抗氧化活性分别提高了3.86%-28.07%和4.21%-9.18%(P<0.05)。这些发现表明,特别是由ACEW介导的发芽可能是通过重建内源性抗氧化系统进一步优化亚麻籽营养和功能特性的有效方法。