Yu Zhang-Long, Liu Rui
1Cotton Research Institute, Shanxi Academy of Agricultural Sciences, No. 118 The Yellow River Avenue, Yanhu, Yuncheng, 044000 People's Republic of China.
2Life Sciences Department, Yuncheng University, No. 1155 Fudan West Street, Yanhu, Yuncheng, 044000 People's Republic of China.
J Food Sci Technol. 2019 Mar;56(3):1495-1501. doi: 10.1007/s13197-019-03637-5. Epub 2019 Feb 13.
Triticale malt can be used as a source of enzymes or as a raw material for the production of functional foods. In this study, triticale malt was produced by soaking triticale seeds either in tap water (TW) or slightly acidic electrolyzed water (SAEW) and then rinsing with TW, SAEW, or alkaline electrolyzed water (AEW). We determined the length of the hypocotyl of triticale malt and the activities of α-amylase, phytase, proteases, and lipase during 4 days of germination. The electrolyzed water (EW) treatments promoted the growth of triticale malt. On the 4th day of germination, the hypocotyl length of triticale malt soaked in TW and watered with SAEW was 24.57% longer than that of triticale malt soaked and watered with TW. The α-amylase, phytase, acidic protease, and lipase activities of triticale malt soaked in SAEW and watered with AEW were high on the 4th germination day (0.11, 1.24 × 10, 0.62, and 0.51 units/mg protein, respectively). The main finding of this study is that the use of EW, especially during the soaking procedure, may be a promising way to obtain triticale malt with high enzyme activity for use in the production of functional foods.
小黑麦麦芽可用作酶的来源或作为生产功能性食品的原料。在本研究中，通过将小黑麦种子浸泡在自来水（TW）或微酸性电解水（SAEW）中，然后用TW、SAEW或碱性电解水（AEW）冲洗来制备小黑麦麦芽。我们测定了小黑麦麦芽在萌发4天期间下胚轴的长度以及α-淀粉酶、植酸酶、蛋白酶和脂肪酶的活性。电解水（EW）处理促进了小黑麦麦芽的生长。在萌发第4天，浸泡在TW中并用SAEW浇水的小黑麦麦芽的下胚轴长度比浸泡并浇TW的小黑麦麦芽长24.57%。在萌发第4天，浸泡在SAEW中并用AEW浇水的小黑麦麦芽的α-淀粉酶、植酸酶、酸性蛋白酶和脂肪酶活性较高（分别为0.11、1.24×10、0.62和0.51单位/毫克蛋白质）。本研究的主要发现是，使用电解水，尤其是在浸泡过程中，可能是获得具有高酶活性的小黑麦麦芽用于生产功能性食品的一种有前景的方法。
