海胆卵中的儿茶酚胺分泌与腺苷酸环化酶激活

Catecholamine secretion and adenylate cyclase activation in sea urchin eggs.

作者信息

Capasso A, Parisi E, De Prisco P, De Petrocellis B

出版信息

Cell Biol Int Rep. 1987 Jun;11(6):457-63. doi: 10.1016/0309-1651(87)90079-8.

Abstract

The role of neurotransmitters in sea-urchin eggs was investigated by studying their effect on adenylate cyclase of the egg membrane. Maximal stimulation of enzyme activity occurs in the presence of dopamine and GTP. 5-hydroxytriptamine, 5-methoxytriptamine and acetylcholine have no effect on activity, despite a decrease in intracellular cAMP level in eggs treated with 5-hydroxytriptamine antagonists as previously reported (Renaud et al., 1983). High-performance liquid chromatography (HPLC) revealed that dopamine is released from the sea-urchin egg into the external medium following fertilization.

摘要

通过研究神经递质对海胆卵细胞膜腺苷酸环化酶的作用，对其在海胆卵中的作用进行了研究。在多巴胺和鸟苷三磷酸（GTP）存在的情况下，酶活性出现最大刺激。5-羟色胺、5-甲氧基色胺和乙酰胆碱对活性没有影响，尽管如先前报道（雷诺等，1983年），用5-羟色胺拮抗剂处理的卵中细胞内环磷酸腺苷（cAMP）水平有所下降。高效液相色谱法（HPLC）显示，受精后多巴胺从海胆卵释放到外部介质中。