College of Life Science, Sichuan Agricultural University, Ya'an, 625014, China.
Food Chem Toxicol. 2021 Dec;158:112667. doi: 10.1016/j.fct.2021.112667. Epub 2021 Nov 8.
Acrylamide (AC), a proved toxin is mainly used in industrial fields and proved to possess various toxicities. In recent years, AC has been found in starch-containing foods due to Maillard reaction in a high-temperature process. Therefore, how to mitigate the toxic effect of AC is a research spot. Blumea laciniata is a widely used folk medicine in Asia and the extract from B. laciniata (EBL) exhibited a strong protection on cells against oxidative stress. In this work, we used EBL to protect Hep G2 cells and Caenorhabditis elegans against AC toxicity. As the results turned out, EBL increased cell viability under AC stress and notably reduced the cell apoptosis through decreasing the high level of ROS. Moreover, EBL extended the survival time of C. elegans, while EBL failed to prolong the survival time of mutants that were in Insulin signaling pathway. Besides, the expressions of antioxidant enzymes were activated after the worms were treated with EBL and daf-16 gene was activated. Our results indicated that EBL exhibited a protective effect against AC induced toxicity in Hep G2 cells and C. elegans via Insulin/IGF-1 signaling pathway. These outcomes may provide a promising natural drug to alleviate the toxic effect of AC.
丙烯酰胺（AC）是一种已被证实的毒素，主要用于工业领域，已被证明具有多种毒性。近年来，由于高温过程中的美拉德反应，在含淀粉的食物中发现了丙烯酰胺。因此，如何减轻丙烯酰胺的毒性作用是一个研究热点。菊三七是亚洲广泛使用的民间药物，其提取物（EBL）对细胞的氧化应激具有很强的保护作用。在这项工作中，我们使用 EBL 来保护 Hep G2 细胞和秀丽隐杆线虫免受 AC 毒性的影响。结果表明，EBL 在 AC 应激下增加了细胞活力，并通过降低高水平的 ROS 显著减少了细胞凋亡。此外，EBL 延长了秀丽隐杆线虫的存活时间，而 EBL 未能延长胰岛素信号通路突变体的存活时间。此外，在用 EBL 处理线虫后，抗氧化酶的表达被激活，daf-16 基因被激活。我们的结果表明，EBL 通过胰岛素/IGF-1 信号通路对 Hep G2 细胞和秀丽隐杆线虫中的 AC 诱导毒性表现出保护作用。这些结果可能为减轻丙烯酰胺的毒性作用提供一种有前途的天然药物。
