State Key Laboratory of Silicon Materials, School of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, People's Republic of China.
Department of Food Science and Nutrition, Zhejiang University, Hangzhou, 310058, People's Republic of China; Zhejiang Key Laboratory for Agro-food Processing, Zhejiang University, Hangzhou 310058, People's Republic of China; Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, People's Republic of China; National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People's Republic of China.
J Hazard Mater. 2020 Jul 5;393:122364. doi: 10.1016/j.jhazmat.2020.122364. Epub 2020 Feb 21.
Acrylamide (ACR), as a raw material of polyacrylamide that used in water purification, was verified to possess various toxicity. Tetrastigma hemsleyanum (TH) is a medicinal plant widely used to anti-inflammation and anti-tumor in Chinese folks. However, more researches focused on the biological activities in tubers and the leaves were ignored. Thus, the protective effect of Tetrastigma hemsleyanum leaves extract (THLE) against ACR-induced toxicity in HepG2 cells and Caenorhabditis elegans (C. elegans) was explored in this study. In vitro, we observed that THLE attenuated ACR-induced toxicity in HepG2 cell via regulating Akt/mTOR/FOXO1/MAPK signaling pathway. Further research proved that 5-caffeoylquinic acid (5-CA) plays a major role in THLE's amelioration effect of ACR toxicity. In vivo, it was found that THLE possesses the same protective effect in ACR-treated wild-type N2 C. elegans and daf-2 (-) (deficit in DAF-2) mutants. However, the anti-ACR toxicity effect of THLE in daf-16 (-) mutants (deficit in DAF-16 that homologous to FOXO family in human) was weakened. Our results indicated that THLE exhibited protective effects against ACR-induced toxicity both in HepG2 cells and C. elegans, while DAF-16/FOXO gene is involved in THLE' protective effect via regulating the expression levels of downstream antioxidant genes.
丙烯酰胺（ACR）作为用于水净化的聚丙烯酰胺的原料，已被证实具有多种毒性。四叶参（TH）是一种药用植物，在中国民间广泛用于抗炎和抗肿瘤。然而，更多的研究集中在块茎上的生物活性，而忽略了叶子。因此，本研究探讨了四叶参叶提取物（THLE）对 HepG2 细胞和秀丽隐杆线虫（C. elegans）中 ACR 诱导毒性的保护作用。在体外，我们观察到 THLE 通过调节 Akt/mTOR/FOXO1/MAPK 信号通路来减轻 HepG2 细胞中 ACR 诱导的毒性。进一步的研究证明，5-咖啡酰奎宁酸（5-CA）在 THLE 减轻 ACR 毒性中起主要作用。在体内，发现 THLE 在 ACR 处理的野生型 N2 C. elegans 和 daf-2（-）（DAF-2 缺陷）突变体中具有相同的保护作用。然而，THLE 在 daf-16（-）突变体（DAF-16 缺陷，与人 FOXO 家族同源）中的抗 ACR 毒性作用减弱。我们的结果表明，THLE 对 HepG2 细胞和 C. elegans 中 ACR 诱导的毒性均表现出保护作用，而 DAF-16/FOXO 基因通过调节下游抗氧化基因的表达水平参与 THLE 的保护作用。
