Oh Hyun-Ji, Jin Heegu, Nah Seung-Yeol, Lee Boo-Yong
Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam, Kyonggi, Republic of Korea.
Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
J Ginseng Res. 2021 Nov;45(6):744-753. doi: 10.1016/j.jgr.2021.07.006. Epub 2021 Jul 23.
Gintonin-enriched fraction (GEF) is a new non-saponin component glycolipoprotein isolated from ginseng root. This study examined the effect of GEF on age-related sarcopenia in old C57BL/6J mice.
Young (3-6 months) and old (20-24 months) C57BL/6J mice received oral GEF (50 mg/kg/day or 150 mg/kg/day) daily for 5 weeks. During the oral administration period, body weight and grip strength were measured weekly. After sacrifice, muscles from the hindlimb were excised and used for hematoxylin and eosin staining and western blotting to determine the effects of GEF on sarcopenia. The thymus was photographed to compare size, and flow cytometry was performed to examine the effect of GEF on immune homeostasis in the thymus and spleen. Blood samples were collected, and the concentrations of pro-inflammatory cytokines and IGF-1 were measured.
GEF caused a significant increase in muscle strength, mass, and fiber size in old mice. GEF restored age-related disruption of immune homeostasis by maintaining T cell compartments and regulating inflammatory biomarkers. Thus, GEF reduced common low-grade chronic inflammatory parameters, which are the main cause of muscle loss.
GEF maintained immune homeostasis and inhibited markers of chronic inflammation, resulting in anti-sarcopenia effects in aged C57BL/6J mice. Thus, GEF is a potential therapeutic agent that slows sarcopenia in the elderly.
人参皂苷富集组分（GEF）是从人参根中分离出的一种新型非皂苷成分糖脂蛋白。本研究检测了GEF对老年C57BL/6J小鼠年龄相关性肌肉减少症的影响。
年轻（3 - 6个月）和老年（20 - 24个月）C57BL/6J小鼠每天口服GEF（50毫克/千克/天或150毫克/千克/天），持续5周。在口服给药期间，每周测量体重和握力。处死后，切除后肢肌肉，用于苏木精和伊红染色及蛋白质印迹分析，以确定GEF对肌肉减少症的影响。拍摄胸腺照片以比较大小，并进行流式细胞术检测GEF对胸腺和脾脏免疫稳态的影响。采集血样，测量促炎细胞因子和IGF - 1的浓度。
GEF使老年小鼠的肌肉力量、质量和纤维大小显著增加。GEF通过维持T细胞区室和调节炎症生物标志物，恢复了与年龄相关的免疫稳态破坏。因此，GEF降低了常见的低度慢性炎症参数，而这些参数是肌肉流失的主要原因。
GEF维持免疫稳态并抑制慢性炎症标志物，从而在老年C57BL/6J小鼠中产生抗肌肉减少症作用。因此，GEF是一种潜在的治疗药物，可减缓老年人的肌肉减少症。
