Lee Rami, Lee Byung-Hwan, Choi Sun-Hye, Cho Yeon-Jin, Cho Han-Sung, Kim Hyoung-Chun, Rhim Hyewhon, Cho Ik-Hyun, Rhee Man Hee, Nah Seung-Yeol
Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
Neuropsychopharmacology and Toxicology program, College of Pharmacy, Kangwon National University, Chunchon, Republic of Korea.
J Ginseng Res. 2021 Sep;45(5):583-590. doi: 10.1016/j.jgr.2021.02.006. Epub 2021 Feb 22.
Gintonin, isolated from ginseng, acts as a ginseng-derived lysophosphatidic acid (LPA) receptor ligand and elicits the [Ca] transient through six LPA receptor subtypes (LPARSs). However, the long-term effects of gintonin-enriched fraction (GEF) on the gene expression of six LPARSs remain unknown. We examined changes in the gene expression of six LPA receptors in the mouse whole brain, heart, lungs, liver, kidneys, spleen, small intestine, colon, and testis after long-term oral GEF administration.
C57BL/6 mice were divided into two groups: control vehicle and GEF (100 mg/kg, ). After 21-day saline or GEF treatment, total RNA was extracted from nine mouse organs. Quantitative-real-time PCR (qRT-PCR) and western blot were performed to quantify changes in the gene and protein expression of the six LPARSs, respectively.
qRT-PCR analysis before GEF treatment revealed that the LPA6 RS was predominant in all organs except the small intestine. The LPA2 RS was most abundant in the small intestine. Long-term GEF administration differentially regulated the six LPARSs. Upon GEF treatment, the LPA6 RS significantly increased in the liver, small intestine, colon, and testis but decreased in the whole brain, heart, lungs, and kidneys. Western blot analysis of the LPA6 RS confirmed the differential effects of GEF on LPA6 receptor protein levels in the whole brain, liver, small intestine, and testis.
The LPA6 receptor was predominantly expressed in all nine organs examined; long-term oral GEF administration differentially regulated LPA3, LPA4, and LPA6 receptors in the whole brain, heart, lungs, liver, kidneys, small intestine, and testis.
从人参中分离出的人参皂草苷，作为一种源自人参的溶血磷脂酸（LPA）受体配体，通过六种LPA受体亚型（LPARSs）引发[Ca]瞬变。然而，富含人参皂草苷的组分（GEF）对六种LPARSs基因表达的长期影响尚不清楚。我们研究了长期口服GEF后，小鼠全脑、心脏、肺、肝脏、肾脏、脾脏、小肠、结肠和睾丸中六种LPA受体基因表达的变化。
将C57BL/6小鼠分为两组：对照载体组和GEF组（100mg/kg）。在进行21天的生理盐水或GEF处理后，从九个小鼠器官中提取总RNA。分别进行定量实时PCR（qRT-PCR）和蛋白质印迹分析，以量化六种LPARSs基因和蛋白质表达的变化。
GEF处理前的qRT-PCR分析显示，除小肠外，LPA6 RS在所有器官中占主导地位。LPA2 RS在小肠中最为丰富。长期给予GEF对六种LPARSs有不同的调节作用。经GEF处理后，肝脏、小肠、结肠和睾丸中的LPA6 RS显著增加，而全脑、心脏、肺和肾脏中的LPA6 RS则减少。对LPA6 RS的蛋白质印迹分析证实了GEF对全脑、肝脏、小肠和睾丸中LPA6受体蛋白水平的不同影响。
LPA6受体在所有九个检测器官中均有主要表达；长期口服GEF对全脑、心脏、肺、肝脏、肾脏、小肠和睾丸中的LPA3、LPA4和LPA6受体有不同的调节作用。
