Lee Na-Eun, Park Sang-Deuk, Hwang Hongik, Choi Sun-Hye, Lee Ra Mi, Nam Sung Min, Choi Jong Hee, Rhim Hyewhon, Cho Ik-Hyun, Kim Hyoung-Chun, Hwang Sung-Hee, Nah Seung-Yeol
Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
Center for Neuroscience, Korea Institute of Science and Technology, Seoul, Republic of Korea.
J Ginseng Res. 2020 Jan;44(1):168-177. doi: 10.1016/j.jgr.2019.05.013. Epub 2019 Jun 7.
Ginseng has been widely used as a health-promoting tonic. Gintonin present in ginseng acts as a lysophosphatidic acid (LPA) receptor ligand that activates six LPA receptor subtypes. The LPA6 subtype plays a key role in normal hair growth, and mutations in the LPA6 receptor impair normal human hair growth. Currently, human hair loss and alopecia are concerning issues that affect peoples' social and day-to-day lives.
We investigated the and effects of a gintonin-enriched fraction (GEF) on mouse hair growth.
Human hair follicle dermal papilla cells (HFDPCs) and six-week-old male C57BL/6 mice were used. The mice were divided into the four groups: control, 1% minoxidil, 0.75% GEF, and 1.5% GEF. The dorsal hair was removed to synchronize the telogen phase. Each group was treated topically, once a day, for 15 days. We analyzed hair growth activity and histological changes.
GEF induced transient [Ca], which stimulated HFDPC proliferation and caused 5-bromo-2'-deoxyuridine (BrdU) incorporation in a concentration-dependent manner. GEF-mediated HFDPC proliferation was blocked by the LPA receptor antagonist and Ca chelator. HFDPC treatment with GEF stimulated vascular endothelial growth factor release. Topical application of GEF and minoxidil promoted hair growth in a dose-dependent manner. Histological analysis showed that GEF and minoxidil increased the number of hair follicles and hair weight.
Topical application of GEF promotes mouse hair growth through HFDPC proliferation. GEF could be one of the main components of ginseng that promote hair growth and could be used to treat human alopecia.
人参作为一种促进健康的滋补品已被广泛使用。人参中含有的ginsenin作为一种溶血磷脂酸（LPA）受体配体，可激活六种LPA受体亚型。LPA6亚型在正常头发生长中起关键作用，LPA6受体的突变会损害正常的人类头发生长。目前，人类脱发是影响人们社交和日常生活的令人担忧的问题。
我们研究了富含ginsenin的组分（GEF）对小鼠头发生长的 和 作用。
使用人毛囊真皮乳头细胞（HFDPCs）和六周龄雄性C57BL/6小鼠。将小鼠分为四组：对照组、1%米诺地尔组、0.75% GEF组和1.5% GEF组。去除背部毛发以使休止期同步。每组每天局部治疗一次，持续15天。我们分析了毛发生长活性和组织学变化。
GEF诱导短暂的[Ca]，刺激HFDPC增殖并以浓度依赖的方式导致5-溴-2'-脱氧尿苷（BrdU）掺入。LPA受体拮抗剂和钙螯合剂阻断了GEF介导的HFDPC增殖。用GEF处理HFDPC刺激血管内皮生长因子释放。局部应用GEF和米诺地尔以剂量依赖的方式促进毛发生长。组织学分析表明，GEF和米诺地尔增加了毛囊数量和毛发重量。
局部应用GEF通过HFDPC增殖促进小鼠毛发生长。GEF可能是人参中促进毛发生长的主要成分之一，可用于治疗人类脱发。
