Abdel-Salam Eslam M, Qahtan Ahmed A, Gaafar Abdel-Rhman Z, Zein El-Abedein Assem I, Alshameri Aref M, Alhamdan Abdullah M
Department of Botany & Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Chair of Dates Industry and Technology, College of Food and Agricultural Sciences, King Saud University, PO Box 2460, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2021 Nov;28(11):6023-6029. doi: 10.1016/j.sjbs.2021.07.006. Epub 2021 Jul 10.
Long non-coding RNAs (lncRNAs) play pivot roles in regulating mRNA expression in eukaryotic organisms without coding any proteins. In the current study, a comprehensive analysis of 260 published RNA-Seq datasets collected from different tissues (fruits, leaves, stems, and roots) of L. was performed to discover potential lncRNAs. A total of 10,564 unique lncRNAs were identified. Our results showed that 77.14% of the lncRNAs were intergenic and 60.39% of them are located within 5 Kbp from the partner gene. In general, all the identified lncRNAs showed shorter lengths, fewer number of exons, and lower expression levels as compared to mRNAs in different studied tissues. Several lncRNAs were determined as differentially expressed (DE) in fruits as compared to leaves, stems, or roots. The functional characterization of the DE lncRNAs revealed their roles in regulating significant biological processes in different tissues of . . The current study provides a comprehensive analysis and dataset of lncRNAs in . that could be utilized in further studies concerning the roles of these molecules in plant cells.
长链非编码RNA（lncRNAs）在真核生物中调节mRNA表达时发挥着关键作用，且不编码任何蛋白质。在本研究中，对从L.的不同组织（果实、叶片、茎和根）收集的260个已发表的RNA-Seq数据集进行了全面分析，以发现潜在的lncRNAs。共鉴定出10564个独特的lncRNAs。我们的结果表明，77.14%的lncRNAs是基因间的，其中60.39%位于距伙伴基因5 Kbp范围内。总体而言，与不同研究组织中的mRNA相比，所有鉴定出的lncRNAs均显示出较短的长度、较少的外显子数量和较低的表达水平。与叶片、茎或根相比，有几个lncRNAs在果实中被确定为差异表达（DE）。DE lncRNAs的功能表征揭示了它们在调节......不同组织中的重要生物学过程中的作用。本研究提供了......中lncRNAs的全面分析和数据集，可用于进一步研究这些分子在植物细胞中的作用。
