Yang Liuqing, Li Lina, Chang Pan, Wei Ming, Chen Jianting, Zhu Chaofan, Jia Jing
Second Affiliated Hospital of Xi'an Medical University, Xi' an, China.
First Department of Medical Oncology, Affiliated Shaanxi Provincial Cancer Hospital, Xi'an, China.
Front Genet. 2021 Oct 26;12:690196. doi: 10.3389/fgene.2021.690196. eCollection 2021.
Gastric cancer is one of the most common malignancies harmful to human health. The search for effective drugs or gene therapy has aroused the attention of scientists. So far, microRNAs, as small non-coding RNAs, have the potential to be therapeutic targets for cancer. Herein, we found a highly expressed miR-25 in gastric cancer cell. However, the function of miR-25 for gastric cancer cell growth and apoptosis was unknown. Functionally, we used RT-qPCR, western blot, CCK-8, and flow cytometry to detect gastric cancer cell growth and apoptosis. The results indicated that miR-25 promoted gastric cancer cell growth and inhibited their apoptosis. Mechanistically, we found that a gene EGR2 was a potential target gene of miR-25. Further dual-luciferase results supported this prediction. Moreover, knockdown of EGR2 promoted gastric cancer cell growth and inhibited their apoptosis by flow cytometry detection. Altogether, these findings revealed miR-25 as a regulator of gastric cancer cell growth and apoptosis through targeting EGR2.
胃癌是对人类健康危害最大的常见恶性肿瘤之一。寻找有效的药物或基因治疗方法已引起科学家们的关注。到目前为止，微小RNA作为小的非编码RNA，有潜力成为癌症的治疗靶点。在此，我们发现胃癌细胞中miR-25高度表达。然而，miR-25对胃癌细胞生长和凋亡的作用尚不清楚。在功能上，我们使用RT-qPCR、蛋白质免疫印迹法、CCK-8法和流式细胞术来检测胃癌细胞的生长和凋亡。结果表明，miR-25促进胃癌细胞生长并抑制其凋亡。从机制上讲，我们发现基因EGR2是miR-25的潜在靶基因。进一步的双荧光素酶结果支持了这一预测。此外，通过流式细胞术检测，敲低EGR2可促进胃癌细胞生长并抑制其凋亡。总之，这些发现揭示了miR-25通过靶向EGR2作为胃癌细胞生长和凋亡的调节因子。
