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通过圆盘电泳对某些锥虫种类进行蛋白质分型,特别着重于克氏锥虫。

Protein typing by disc electrophoresis of some species of trypanosomes with special emphasis to Trypanosoma cruzi.

作者信息

Ebert F, Schudnagis R, Mühlpfordt H

出版信息

Tropenmed Parasitol. 1978 Mar;29(1):115-8.

PMID:347650
Abstract

Proteins extracted from culture forms of T. cruzi, T. cruzi-like strains, T. rangeli, T. conhorrini and T. dionisii were separated by disc electrophoresis. The electrophoretic protein patterns of all strains examined were highly reproducible. The results indicate that each of these strains have their own "fingerprints" and the trypanosoma species could be identified by some typical specific protein bands. However, it was not possible to distinguish between the human strains of T. cruzi and the T. cruzi-like strains isolated from other sources.

摘要

从克氏锥虫、类克氏锥虫菌株、朗氏锥虫、康氏锥虫和狄氏锥虫的培养形态中提取的蛋白质,通过圆盘电泳进行分离。所有检测菌株的电泳蛋白质图谱具有高度可重复性。结果表明,这些菌株中的每一种都有其自身的“指纹图谱”,并且锥虫物种可通过一些典型的特异性蛋白条带进行鉴定。然而,无法区分克氏锥虫的人类菌株和从其他来源分离的类克氏锥虫菌株。

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