Schottelius J, Koch O, Uhlenbruck G
Tropenmed Parasitol. 1983 Jun;34(2):89-92.
Four-days-old culture forms of Trypanosoma cruzi (strain Téhuantépéc, Guatemala) and Trypanosoma vespertilionis (strain P-14, P-9) were tested by 19 carbohydrate-specific agglutinins. The T. cruzi strains are interspecifically distinguishable with the lectins from Euonymus europaeus, Tridacna crocea, Tridacna maxima and the human blood-group testserum anti-B from the T. vespertilionis strains. While the T. vespertilionis strains did react with anti-B and E. europaeus, the T. cruzi strains did not agglutinate. The T. cruzi strains were agglutinated by the lectins from T. crocea and T: maxima while the bat-trypanosomes showed no reactions. Using these lectins it was not possible to distinguish the bat-flagellates intraspecifically. With the lectins from Triticum vulgaris and Arachis hypogaea the T. cruzi strains could be distinguished. While the Ténuantépéc strain did agglutinate with A. hypogaea, T. cruzi strain Guatemala did react only with the lectin from T. vulgaris. The bat-trypanosomes were agglutinated only by A. hypogaea but not by T. vulgaris. The reactions of these trypanosome-species with A. papillata and T. vulgaris demonstrate that both trypanosome species have N-acetylneuraminic acid on their cell surfaces.
用19种碳水化合物特异性凝集素对4日龄的克氏锥虫（特万特佩克菌株，危地马拉）和蝙蝠锥虫（P - 14、P - 9菌株）培养形式进行了检测。克氏锥虫菌株与来自欧洲卫矛、大砗磲、长砗磲的凝集素以及人血型抗B血清可在种间与蝙蝠锥虫菌株区分开来。蝙蝠锥虫菌株能与抗B和欧洲卫矛发生反应，而克氏锥虫菌株不发生凝集。克氏锥虫菌株可被来自大砗磲和长砗磲的凝集素凝集，而蝙蝠锥虫则无反应。利用这些凝集素无法在种内区分蝙蝠鞭毛虫。用来自普通小麦和花生的凝集素可区分克氏锥虫菌株。特万特佩克菌株能与花生凝集素发生凝集，而危地马拉克氏锥虫菌株仅与来自普通小麦的凝集素发生反应。蝙蝠锥虫仅被花生凝集素凝集，而不被普通小麦凝集素凝集。这些锥虫物种与乳头艾美耳球虫和普通小麦的反应表明，两种锥虫物种的细胞表面都有N - 乙酰神经氨酸。
