Schottelius J, Müller V
Acta Trop. 1984 Mar;41(1):29-38.
Four-day-old epimastigote culture forms of Trypanosoma cruzi, Trypanosoma rangeli and Trypanosoma conorhini were tested with 21 lectins. Furthermore T. conorhini was incubated with the following sera: rat, Wistar HAN, germ free; normal fresh hen, rat and human serum. T. rangeli was agglutinated only by the D-mannose specific lectins from Canavalia ensiformis and Pisum sativum. T. cruzi and T. conorhini could be distinguished by the lectin from Tridacna crocea. The epimastigote culture forms of T. conorhini were not lysed by normal fresh rat, hen and human sera. Therefore, T. cruzi, T. conorhini and T. rangeli can be distinguished interspecifically by lectins and by the different lytic effect of rat, hen and human sera. It is possible to separate each of the species by complement lysis. The lysis-resistant species can be cultivated for further examinations.
用21种凝集素对克氏锥虫、兰氏锥虫和康氏锥虫4日龄的上鞭毛体培养形式进行了检测。此外，将康氏锥虫与以下血清进行孵育：大鼠血清，Wistar HAN大鼠无菌血清；正常新鲜母鸡血清、大鼠血清和人血清。兰氏锥虫仅被来自刀豆和豌豆的D-甘露糖特异性凝集素凝集。克氏锥虫和康氏锥虫可以通过来自大砗磲的凝集素区分开来。康氏锥虫的上鞭毛体培养形式不会被正常新鲜大鼠血清、母鸡血清和人血清溶解。因此，克氏锥虫、康氏锥虫和兰氏锥虫可以通过凝集素以及大鼠、母鸡和人血清的不同溶解作用进行种间区分。通过补体溶解可以分离出每个物种。对具有溶解抗性的物种可以进行培养以作进一步检测。
