Saki Jasem, Zamanpour Maryam, Najafian Mahin, Mohammadpour Niloofar, Foroutan Masoud
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Medical Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Parasitol Res. 2021 Nov 2;2021:6693070. doi: 10.1155/2021/6693070. eCollection 2021.
() is one of the most common intracellular protozoan parasites, which can infect humans and a wide range of mammals and birds. The current study is aimed at investigating the occurrence of infection in women with a history of abortion in Khuzestan, Iran.
A total of 480 women with an abortion history, as well as 200 pregnant women with a normal delivery, were examined in this study. The blood, placenta, and umbilical cord blood samples were assessed by the enzyme-linked immunosorbent assay (ELISA) and nested-polymerase chain reaction (PCR) assay.
Based on the results of ELISA assay, the prevalence of toxoplasmosis was 30.83% in women with a history of abortion (25.62% with IgG and 5.20% with IgM). According to the IgG avidity test, 60.16% of IgG-positive samples showed high avidity, while 27.64% showed low avidity. On the other hand, the prevalence of toxoplasmosis in women with a normal delivery was 23% (21.5% with IgG and 1.5% with IgM). According to the IgG avidity test, 81.39% of these women showed high avidity, while only 4.65% showed low avidity. Based on the nested-PCR method, DNA was detected in 14.18% of blood samples, 4.69% of placental samples, and 1.34% of umbilical cord samples, collected from 148 seropositive women with a history of abortion. Besides, using this method, the parasite DNA was identified in 4.34% of blood samples, collected from 46 seropositive women with a normal delivery, but not in any of the umbilical cord or placenta samples.
The present results showed that infection contributes to abortion in Khuzestan Province, Iran. Therefore, it is essential to investigate toxoplasmosis in pregnant women, especially in those who are seronegative, using molecular and serological methods and inform them about their disease and the associated risks.
（某种寄生虫）是最常见的细胞内原生动物寄生虫之一，可感染人类以及多种哺乳动物和鸟类。当前研究旨在调查伊朗胡齐斯坦省有流产史女性中该寄生虫感染的发生情况。
本研究共检查了480名有流产史的女性以及200名正常分娩的孕妇。通过酶联免疫吸附测定（ELISA）和巢式聚合酶链反应（PCR）测定对血液、胎盘和脐带血样本进行评估。
基于ELISA测定结果，有流产史女性中弓形虫病的患病率为30.83%（IgG阳性率为25.62%，IgM阳性率为5.20%）。根据IgG亲和力检测，60.16%的IgG阳性样本显示高亲和力，而27.64%显示低亲和力。另一方面，正常分娩女性中弓形虫病的患病率为23%（IgG阳性率为21.5%，IgM阳性率为1.5%）。根据IgG亲和力检测，这些女性中81.39%显示高亲和力，而仅4.65%显示低亲和力。基于巢式PCR方法，在从148名有流产史的血清阳性女性采集的血液样本中，14.18%检测到该寄生虫DNA，胎盘样本中4.69%检测到，脐带样本中1.34%检测到。此外，使用该方法，在从46名正常分娩的血清阳性女性采集的血液样本中，4.34%鉴定出寄生虫DNA，但在任何脐带或胎盘样本中均未检测到。
目前结果表明，在伊朗胡齐斯坦省，该寄生虫感染与流产有关。因此，至关重要的是使用分子和血清学方法对孕妇，尤其是血清阴性的孕妇进行弓形虫病调查，并告知她们所患疾病及相关风险。
