Ignatova Lyudmila, Kistaubayeva Aida, Brazhnikova Yelena, Omirbekova Anel, Mukasheva Togzhan, Savitskaya Irina, Karpenyuk Tatyana, Goncharova Alla, Egamberdieva Dilfuza, Sokolov Alexander
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, 050038, Kazakhstan.
Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg, Germany.
Heliyon. 2021 Oct 22;7(11):e08240. doi: 10.1016/j.heliyon.2021.e08240. eCollection 2021 Nov.
Cadmium stress disrupts plant-microbial interactions and reduces plant growth and development. In plants, the tolerance to stress can be increased by inoculation with endophytic microorganisms. The aim of this study was to investigate the distribution of endophytic fungi in various plant organs of barley and soybean and evaluate their Cd removal ability. Two hundred fifty-three fungal strains were isolated from various organs of barley ( cv Arna) and soybean ( cv Almaty). The colonization rate ranged from 13.6% to 57.3% and was significantly higher in the roots. Ten genera were identified: and . Twenty-three fungal strains have a Cd tolerance index from 0.24 to 1.12. Five strains ( T7, T15, MK1, RH2, MP2) with the highest level of Cd tolerance have minimum inhibitory concentrations from 290 to 2400 μg/ml. These fungi were able to remove Cd up to 59%. The bioaccumulation capacity ranged from 2.3 to 11.9 mg/g. Selected fungal strains could be considered as biological agents for their potential application in the bioremediation of contaminated sites.
镉胁迫会破坏植物与微生物的相互作用，并降低植物的生长发育。在植物中，接种内生微生物可提高其对胁迫的耐受性。本研究的目的是调查大麦和大豆各植物器官中内生真菌的分布，并评估它们的镉去除能力。从大麦（品种Arna）和大豆（品种阿拉木图）的各个器官中分离出253株真菌菌株。定殖率在13.6%至57.3%之间，且在根部显著更高。鉴定出了10个属：[此处原文缺失具体属名]。23株真菌菌株的镉耐受指数为0.24至1.12。五株镉耐受性最高的菌株（T7、T15、MK1、RH2、MP2）的最低抑菌浓度为290至2400μg/ml。这些真菌能够去除高达59%的镉。生物积累能力在2.3至11.9mg/g之间。所选真菌菌株因其在污染场地生物修复中的潜在应用而可被视为生物制剂。
