Chandel Ankush, Mann Ross, Kaur Jatinder, Tannenbaum Ian, Norton Sally, Edwards Jacqueline, Spangenberg German, Sawbridge Timothy
Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, VIC, 3083, Australia.
School of Applied Systems Biology, La Trobe University, Bundoora, VIC, 3083, Australia.
Environ Microbiome. 2022 Nov 16;17(1):56. doi: 10.1186/s40793-022-00452-y.
Plant microbiome composition has been demonstrated to change during the domestication of wild plants and it is suggested that this has resulted in loss of plant beneficial microbes. Recently, the seed microbiome of native plants was demonstrated to harbour a more diverse microbiota and shared a common core microbiome with modern cultivars. In this study the composition of the seed-associated bacteria of Glycine clandestina is compared to seed-associated bacteria of Glycine max (soybean).
The seed microbiome of the native legume Glycine clandestina (crop wild relative; cwr) was more diverse than that of the domesticated Glycine max and was dominated by the bacterial class Gammaproteobacteria. Both the plant species (cwr vs domesticated) and individual seed accessions were identified as the main driver for this diversity and composition of the microbiota of all Glycine seed lots, with the effect of factor "plant species" exceeded that of "geographical location". A core microbiome was identified between the two Glycine species. A high percentage of the Glycine microbiome was unculturable [G. clandestina (80.8%) and G. max (75.5%)] with only bacteria of a high relative abundance being culturable under the conditions of this study.
Our results provided novel insights into the structure and diversity of the native Glycine clandestina seed microbiome and how it compares to that of the domesticated crop Glycine max. Beyond that, it also increased our knowledge of the key microbial taxa associated with the core Glycine spp. microbiome, both wild and domesticated. The investigation of this commonality and diversity is a valuable and essential tool in understanding the use of native Glycine spp. for the discovery of new microbes that would be of benefit to domesticated Glycine max cultivars or any other economically important crops. This study has isolated microbes from a crop wild relative that are now available for testing in G. max for beneficial phenotypes.
植物微生物组的组成在野生植物驯化过程中已被证明会发生变化，有人认为这导致了植物有益微生物的丧失。最近，已证明本地植物的种子微生物组含有更多样化的微生物群，并与现代栽培品种共享一个共同的核心微生物组。在本研究中，将野生大豆（Glycine clandestina）种子相关细菌的组成与栽培大豆（Glycine max）种子相关细菌进行了比较。
本地豆科植物野生大豆（作物野生近缘种；cwr）的种子微生物组比栽培大豆的更具多样性，且以γ-变形菌纲细菌为主。植物物种（cwr与栽培种）和单个种子种质均被确定为所有大豆种子批次微生物群这种多样性和组成的主要驱动因素，“植物物种”因素的影响超过了“地理位置”。在这两种大豆物种之间鉴定出了一个核心微生物组。大豆微生物组中有很大比例是不可培养的[野生大豆（80.8%）和栽培大豆（75.5%）]，在本研究条件下，只有相对丰度高的细菌才可培养。
我们的结果为野生大豆种子微生物组的结构和多样性以及它与栽培作物大豆的比较提供了新的见解。除此之外，它还增加了我们对与野生和栽培大豆核心微生物组相关的关键微生物类群的了解。对这种共性和多样性的研究是理解利用本地大豆物种发现对栽培大豆品种或任何其他经济上重要作物有益的新微生物的宝贵且必不可少的工具。本研究已从一种作物野生近缘种中分离出微生物，现在可在栽培大豆中测试其有益表型。
