School of Medicine, Mae Fah Luang University, Chiang Rai, Thailand.
Cancer Research Unit of Associated Medical Sciences (AMS-CRU), Faculties of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
Nutr Cancer. 2022;74(6):2254-2264. doi: 10.1080/01635581.2021.2002921. Epub 2021 Nov 12.
Inflammatory response facilitating colorectal cancer (CRC) progression is a serious event following operative infection, which can occur in CRC patients. This event is mainly mediated by bacterial lipopolysaccharide (LPS), via a toll like receptor 4 (TLR4) and NF-κB. Hexane soluble fraction (HSF) from purple rice extract (PRE) has been identified as a γ-oryzanol (OR)-rich fraction. Recently, HSF possessed inhibitory effect of LPS-stimulated metastasis of human colon cancer SW480 cells, however the related mechanism was unknown. Thus, this study aimed to investigate the effect of HSF on inflammatory response-associated cancer progression of LPS-stimulated SW480 cells. The various inflammatory mediators, vascular endothelial growth factor-A (VEGFA) and related pathways were evaluated by Western blot and ELISA. Furthermore, cell migration was also determined by migration assays. Of all, HSF seemed to be stronger than OR to attenuate the responsiveness of LPS on various inflammatory mediators, which was related to an obvious reduction of cancer cell migration as well as indistinct disruption on VEGFA production in SW480 cells, via downregulation of TLR4 and NF-κB. Therefore, OR-rich fraction from PRE, against the subsequent inflammatory response and CRC progression following surgery, which could be combined with conventional treatments to increase the survival rate.
炎症反应促进结直肠癌（CRC）的进展是手术后感染的严重事件，可能发生在 CRC 患者中。该事件主要由细菌脂多糖（LPS）通过 Toll 样受体 4（TLR4）和 NF-κB 介导。已鉴定出紫色稻米提取物（PRE）的己烷可溶部分（HSF）为富含 γ-谷维素（OR）的部分。最近，HSF 具有抑制 LPS 刺激的人结肠癌细胞 SW480 细胞转移的作用，但相关机制尚不清楚。因此，本研究旨在探讨 HSF 对 LPS 刺激的 SW480 细胞炎症反应相关癌症进展的影响。通过 Western blot 和 ELISA 评估了各种炎症介质、血管内皮生长因子-A（VEGFA）和相关途径。此外，还通过迁移实验测定了细胞迁移。总之，HSF 似乎比 OR 更强，可减弱 LPS 对各种炎症介质的反应性，这与 SW480 细胞中明显降低的癌细胞迁移以及 VEGFA 产生的不明显破坏有关，这是通过下调 TLR4 和 NF-κB 实现的。因此，PRE 中的 OR 丰富部分可预防手术后的炎症反应和 CRC 进展，可与常规治疗联合使用，以提高生存率。
