Department of Biosystems Science and Engineering, ETH Zurich, Switzerland.
Department of Biosystems Science and Engineering, ETH Zurich, Switzerland.
Metab Eng. 2022 Jan;69:98-111. doi: 10.1016/j.ymben.2021.10.014. Epub 2021 Nov 10.
The biosynthesis of short-chain alcohols is a carbon-neutral alternative to petroleum-derived production, but strain screening operations are encumbered by laborious analytics. Here, we built, characterized and applied whole cell biosensors by directed evolution of the transcription factor AlkS for screening microbial strain libraries producing industrially relevant alcohols. A selected AlkS variant was applied for in situ product detection in two screening applications concerning key steps in alcohol production. Further, the biosensor strains enabled the implementation of an automated, robotic platform-based workflow with data clustering, which readily allowed the identification of significantly improved strain variants for isopentanol production.
短链醇的生物合成是一种对石油衍生生产的碳中性替代方法，但菌株筛选操作受到繁琐的分析的阻碍。在这里，我们通过定向进化转录因子 AlkS 构建、表征和应用了全细胞生物传感器，用于筛选生产工业相关醇的微生物菌株文库。选择的 AlkS 变体用于原位产品检测，应用于两个与醇生产关键步骤相关的筛选应用中。此外，该生物传感器菌株还实现了一个自动化、基于机器人平台的工作流程，并进行数据聚类，这可以很容易地识别出异戊醇生产中显著改进的菌株变体。
