Hiddemann W, Harbott J, Haas H O, Budde M, Büchner T, Lampert F
Klin Padiatr. 1987 May-Jun;199(3):161-4. doi: 10.1055/s-2008-1026782.
In the present study a comparative analysis of DNA flow cytometry and cytogenetics was performed in 257 children with acute leukemias to evaluate the comparability of both techniques for the detection of karyotype aberrations. DNA aneuploidies were detected in 88 (34%) patients while cytogenetic anomalies were revealed in 96 (37%) cases. From 76 patients (30%) with cytogenetically inevaluable metaphases 26 (35%) expressed aneuploid DNA stemlines. 67 (79%) of the 85 patients with normal karyotypes or normal modal chromosome numbers had a DNA index of 1.0 while DNA aneuploidies were identified in 18 cases (21%). 4 of 30 patients (13%) with pseudodiploid karyotypes revealed aneuploid DNA stemlines. While aberrations of the chromosome number by 1-3 chromosomes were combined with DNA aneuploidies in 9 of 35 cases (26%) only, all 31 patients with higher numeric chromosome changes also had aneuploid DNA stemlines. These data indicate that cytogenetics and DNA flow cytometry are complimentary methods for the detection of karyotype aberrations and should therefore be applied simultaneously.
在本研究中，对257例急性白血病患儿进行了DNA流式细胞术和细胞遗传学的比较分析，以评估这两种技术在检测核型畸变方面的可比性。88例（34%）患者检测到DNA非整倍体，96例（37%）病例发现细胞遗传学异常。在76例（30%）细胞遗传学上无法评估中期分裂相的患者中，26例（35%）表现出非整倍体DNA干系。85例核型正常或染色体众数正常的患者中，67例（79%）的DNA指数为1.0，18例（21%）鉴定出DNA非整倍体。30例假二倍体核型患者中有4例（13%）显示非整倍体DNA干系。虽然在35例中的9例（26%）中，染色体数目增减1 - 3条与DNA非整倍体相关，但所有31例染色体数目有更高变化的患者也都有非整倍体DNA干系。这些数据表明，细胞遗传学和DNA流式细胞术是检测核型畸变的互补方法，因此应同时应用。
