Niazy Naima, Mrozek Linus, Barth Mareike, Immohr Moritz Benjamin, Kalampokas Nikolaos, Saeed Diyar, Aubin Hug, Sugimura Yukiharu, Westenfeld Ralf, Boeken Udo, Lichtenberg Artur, Akhyari Payam
Department of Cardiac Surgery, Medical Faculty and University Hospital Düsseldorf, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany.
Department of Cardiac Surgery, Leipzig Heart Center, 04289 Leipzig, Germany.
J Clin Med. 2021 Oct 22;10(21):4856. doi: 10.3390/jcm10214856.
Serum levels of cytokines interleukin 1 beta ( ) and interleukin 33 (IL-33) are highly abnormal in heart failure and remain elevated after mechanical circulatory support (MCS). However, local cytokine signaling induction remains elusive. Left (LV) and right ventricular (RV) myocardial tissue specimens of end-stage heart failure (HF) patients without ( = 24) and with MCS ( = 39; 594 ± 57 days) were analyzed for cytokine mRNA expression level of , interleukin 1 receptor 1/2 (), interleukin 1 receptor-like 1 (), and interleukin-1 receptor accessory protein (). MCS patients showed significantly elevated expression levels (LV: 2.0 fold, = 0.0058; RV: 3.3 fold, < 0.0001). Moreover, , and expression levels strongly correlated with each other. IL-1RL1 and expression levels were significantly higher in RV myocardial tissue (RV/LV ratio HF: 4.400 ± 1.359; MCS: 4.657 ± 0.655; IL-1RL1 HF: 3.697 ± 0.876; MCS: 4.529 ± 0.5839). In addition, IL1-RaP and RV expression levels were significantly elevated in MCS. Furthermore, expression correlates with C-reactive protein (CRP) plasma levels in HF, but not in MCS patients. Increased expression of and altered correlation patterns of receptors indicate enhanced signaling in MCS patients. Correlation of receptor expression with may hint towards a link between both pathways. Moreover, diverging expression in LV and RV suggests specific regulation of local cytokine signaling.
心力衰竭患者血清中细胞因子白细胞介素1β（ ）和白细胞介素33（IL-33）水平高度异常，且在机械循环支持（MCS）后仍持续升高。然而，局部细胞因子信号传导诱导机制仍不清楚。对未接受MCS（ = 24）和接受MCS（ = 39；594 ± 57天）的终末期心力衰竭（HF）患者的左心室（LV）和右心室（RV）心肌组织样本进行分析，检测 、白细胞介素1受体1/2（ ）、白细胞介素1受体样1（ ）和白细胞介素-1受体辅助蛋白（ ）的细胞因子mRNA表达水平。接受MCS的患者 表达水平显著升高（左心室：2.0倍， = 0.0058；右心室：3.3倍， < 0.0001）。此外， 、 和 表达水平彼此之间密切相关。右心室心肌组织中IL-1RL1和 表达水平显著更高（右心室/左心室比值 心力衰竭：4.400 ± 1.359；MCS：4.657 ± 0.655；IL-1RL1 心力衰竭：3.697 ± 0.876；MCS：4.529 ± 0.5839）。此外，MCS患者右心室中IL1-RaP和 表达水平显著升高。此外， 表达与心力衰竭患者血浆中C反应蛋白（CRP）水平相关，但与接受MCS的患者无关。 表达增加以及 受体相关模式改变表明MCS患者中 信号增强。 受体表达与 的相关性可能暗示两条途径之间存在联系。此外，左心室和右心室中表达的差异表明局部细胞因子信号传导存在特定调节。
