Jasemi Seyedesomaye, Erre Gian Luca, Cadoni Maria Luisa, Bo Marco, Sechi Leonardo A
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43b, 07100 Sassari, Italy.
Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy.
J Clin Med. 2021 Nov 2;10(21):5153. doi: 10.3390/jcm10215153.
BACKGROUND/OBJECTIVE: Chronic humoral immune response against multiple microbial antigens may play a crucial role in the etiopathogenesis of rheumatoid arthritis (RA). We aimed to assess the prevalence and magnitude of antibody response against various bacterial and viral immunogen peptides in the sera of RA patients compared with the general population.
Polyclonal IgG antibodies (Abs) specific for peptides derived from (RgpA, Kpg), (LtxA1, LtxA2), subsp. (MAP4027), Epstein-Barr virus (EBNA1, EBVBOLF), and human endogenous retrovirus (HERV-W env-su) were detected by ELISA in serum samples from 148 consecutive RA patients and 148 sex and age-matched healthy controls (HCs). In addition, the presence of a relationship between the positivity and the titer of antibodies and RA descriptors was explored by bivariate correlation analysis.
RA patients exhibit a higher prevalence of humoral immune response against all tested peptides compared to HCs with a statically significant difference for MAP4027 (30.4% vs. 10.1%), BOLF (25.7% vs. 8.1%), RgpA (24.3% vs. 9.4%), HERV W-env (20.3% vs. 9.4%), and EBNA1 (18.9% vs. 9.4%) peptides. Fifty-three (35.8%) out of 148 RA serum and 93 (62.8%) out of 148 HCs were negative for all pathogen-derived peptides. There was a significant correlation between OD values obtained by ELISA test against all peptides ( < 0.0001). We also found an increased titer and prevalence of Abs against LtxA1 and LtxA2 in seropositive vs. seronegative RF ( = 0.019, = 0.018).
This study demonstrates a significantly increased humoral response against multiple pathogens in patients with RA and implies that they could be an important factor in the pathogenesis of the disease. Therefore, the role of each individual pathogen in RA needs to be further investigated.
背景/目的：针对多种微生物抗原的慢性体液免疫反应可能在类风湿关节炎（RA）的发病机制中起关键作用。我们旨在评估与普通人群相比，RA患者血清中针对各种细菌和病毒免疫原肽的抗体反应的患病率和强度。
通过酶联免疫吸附测定（ELISA）检测了148例连续的RA患者和148例性别和年龄匹配的健康对照（HCs）血清样本中针对源自牙龈卟啉单胞菌（RgpA、Kpg）、产单核细胞李斯特菌（LtxA1、LtxA2）、结核分枝杆菌亚种（MAP4027）、爱泼斯坦-巴尔病毒（EBNA1、EBVBOLF）和人类内源性逆转录病毒（HERV-W env-su）的肽的多克隆IgG抗体（Abs）。此外，通过双变量相关分析探讨了抗体阳性和滴度与RA描述符之间的关系。
与HCs相比，RA患者对所有测试肽的体液免疫反应患病率更高，其中针对MAP4027（30.4%对10.1%）、BOLF（25.7%对8.1%）、RgpA（24.3%对9.4%）、HERV W-env（20.3%对9.4%）和EBNA1（18.9%对9.4%）肽的差异具有统计学意义。148份RA血清中有53份（35.8%），148份HCs中有93份（62.8%）对所有病原体衍生肽均呈阴性。ELISA检测针对所有肽获得的OD值之间存在显著相关性（P<0.0001）。我们还发现，血清阳性与血清阴性类风湿因子（RF）患者中，针对LtxA1和LtxA2的Abs滴度和患病率增加（P = 0.019，P = 0.018）。
本研究表明RA患者针对多种病原体的体液反应显著增加，这意味着它们可能是该疾病发病机制中的一个重要因素。因此，每种病原体在RA中的作用需要进一步研究。
