Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2021 Oct 29;22(21):11721. doi: 10.3390/ijms222111721.
Juvenile hormone (JH) plays a critical role in many physiological activities of Arthropoda. Juvenile hormone acid methyltransferase (JHAMT) is involved in the last steps of JH biosynthesis as an important rate-limiting enzyme. In recent studies, an increasing number of JHAMTs were identified in arthropods, but no JHAMT was reported in spiders. Herein, eight JHAMTs were identified in the pond wolf spider, , all containing the well conserved S-adenosyl-L-methionine binding motif. and the other seven were located at chromosome 13 and chromosome 1, respectively. Multiple alignment and phylogenetic analysis showed that JHAMT-1 was grouped together with insect JHAMTs independently and shared high similarities with insect JHAMTs compared to the other seven JHAMTs. In addition, , , and were highly expressed in the abdomen of spiderlings and could respond to the stimulation of exogenous farnesoic acid. Meanwhile, knockdown of these three caused the overweight and accelerated molting of spiderlings. These results demonstrated the cooperation of multi-JHAMTs in spider development and provided a new evolutionary perspective of the expansion of in Arachnida.
保幼激素（JH）在节肢动物的许多生理活动中起着关键作用。保幼激素酸甲基转移酶（JHAMT）作为一种重要的限速酶，参与 JH 生物合成的最后步骤。在最近的研究中，越来越多的 JHAMTs 在节肢动物中被鉴定出来，但在蜘蛛中尚未报道。本文在池塘狼蛛中鉴定出 8 种 JHAMTs，它们都含有保守的 S-腺苷-L-甲硫氨酸结合基序。和其他 7 种分别位于染色体 13 和染色体 1 上。多重比对和系统发育分析表明，JHAMT-1 与昆虫 JHAMTs 独立分组，与其他 7 种 JHAMTs 相比，与昆虫 JHAMTs 具有高度相似性。此外，、、和在幼蛛的腹部中高表达，并能对外源法呢酸的刺激做出反应。同时，这三个的敲低导致幼蛛超重和蜕皮加速。这些结果表明多 JHAMTs 在蜘蛛发育过程中的协同作用，并为蛛形纲动物中 扩张的新进化视角提供了证据。
