对莱姆病的肩突硬蜱传播媒介的基因组学见解。
Genomic insights into the Ixodes scapularis tick vector of Lyme disease.
作者信息
Gulia-Nuss Monika, Nuss Andrew B, Meyer Jason M, Sonenshine Daniel E, Roe R Michael, Waterhouse Robert M, Sattelle David B, de la Fuente José, Ribeiro Jose M, Megy Karine, Thimmapuram Jyothi, Miller Jason R, Walenz Brian P, Koren Sergey, Hostetler Jessica B, Thiagarajan Mathangi, Joardar Vinita S, Hannick Linda I, Bidwell Shelby, Hammond Martin P, Young Sarah, Zeng Qiandong, Abrudan Jenica L, Almeida Francisca C, Ayllón Nieves, Bhide Ketaki, Bissinger Brooke W, Bonzon-Kulichenko Elena, Buckingham Steven D, Caffrey Daniel R, Caimano Melissa J, Croset Vincent, Driscoll Timothy, Gilbert Don, Gillespie Joseph J, Giraldo-Calderón Gloria I, Grabowski Jeffrey M, Jiang David, Khalil Sayed M S, Kim Donghun, Kocan Katherine M, Koči Juraj, Kuhn Richard J, Kurtti Timothy J, Lees Kristin, Lang Emma G, Kennedy Ryan C, Kwon Hyeogsun, Perera Rushika, Qi Yumin, Radolf Justin D, Sakamoto Joyce M, Sánchez-Gracia Alejandro, Severo Maiara S, Silverman Neal, Šimo Ladislav, Tojo Marta, Tornador Cristian, Van Zee Janice P, Vázquez Jesús, Vieira Filipe G, Villar Margarita, Wespiser Adam R, Yang Yunlong, Zhu Jiwei, Arensburger Peter, Pietrantonio Patricia V, Barker Stephen C, Shao Renfu, Zdobnov Evgeny M, Hauser Frank, Grimmelikhuijzen Cornelis J P, Park Yoonseong, Rozas Julio, Benton Richard, Pedra Joao H F, Nelson David R, Unger Maria F, Tubio Jose M C, Tu Zhijian, Robertson Hugh M, Shumway Martin, Sutton Granger, Wortman Jennifer R, Lawson Daniel, Wikel Stephen K, Nene Vishvanath M, Fraser Claire M, Collins Frank H, Birren Bruce, Nelson Karen E, Caler Elisabet, Hill Catherine A
机构信息
Department of Entomology, Purdue University, West Lafayette, Indiana 47907, USA.
Department of Biological Sciences, Old Dominion University, Norfolk, Virginina 23529, USA.
出版信息
Nat Commun. 2016 Feb 9;7:10507. doi: 10.1038/ncomms10507.
Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of the tick, Ixodes scapularis (Say), which vectors pathogens that cause Lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. The large genome reflects accumulation of repetitive DNA, new lineages of retro-transposons, and gene architecture patterns resembling ancient metazoans rather than pancrustaceans. Annotation of scaffolds representing ∼57% of the genome, reveals 20,486 protein-coding genes and expansions of gene families associated with tick-host interactions. We report insights from genome analyses into parasitic processes unique to ticks, including host 'questing', prolonged feeding, cuticle synthesis, blood meal concentration, novel methods of haemoglobin digestion, haem detoxification, vitellogenesis and prolonged off-host survival. We identify proteins associated with the agent of human granulocytic anaplasmosis, an emerging disease, and the encephalitis-causing Langat virus, and a population structure correlated to life-history traits and transmission of the Lyme disease agent.
蜱虫传播给人类和动物的病原体比任何其他节肢动物都多。我们描述了肩突硬蜱（Ixodes scapularis，Say）的21亿碱基对核基因组，该蜱虫是导致莱姆病、人类粒细胞无形体病、巴贝斯虫病和其他疾病的病原体的传播媒介。这个庞大的基因组反映了重复DNA的积累、逆转录转座子的新谱系以及类似于古代后生动物而非泛甲壳动物的基因结构模式。对占基因组约57%的支架的注释揭示了20486个蛋白质编码基因以及与蜱虫-宿主相互作用相关的基因家族的扩展。我们报告了基因组分析对蜱虫特有的寄生过程的见解，包括宿主“搜寻”、长时间进食、角质层合成、血餐浓缩、血红蛋白消化的新方法、血红素解毒、卵黄发生以及长时间的非宿主存活。我们鉴定了与人类粒细胞无形体病（一种新发疾病）的病原体、导致脑炎的兰加特病毒相关的蛋白质，以及与生活史特征和莱姆病病原体传播相关联的种群结构。
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