Zhou Tao, Ye Meiyan, Liu Tianyao, Lan Hujiao, Hashimi Said Masoud, Guo Wei, Liu Jianzhong
Institute of Plant Genetics and Developmental Biology, College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants, College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Feb 25;39(2):586-602. doi: 10.13345/j.cjb.220545.
Autophagy is a highly conserved mechanism for material degradation and recycling in eukaryote cells, and plays important roles in growth, development, stress tolerance and immune responses. ATG10 plays a key role in autophagosome formation. To understand the function of ATG10 in soybean, two homologous genes, namely and , were silenced simultaneously by bean pod mottle virus (BPMV) induced gene silencing. The carbon starvation induced by dark treatment and Western blotting analysis of ATG8 accumulation level indicated that concurrent silencing resulted in the impairment of autophagy in soybean; disease resistance and kinase assays demonstrated that participated in the immune responses by negatively regulating the activation of MPK3/6, indicating that plays a negative regulatory role in immune response in soybean.
自噬是真核细胞中一种高度保守的物质降解和循环利用机制，在生长、发育、胁迫耐受性和免疫反应中发挥重要作用。ATG10在自噬体形成中起关键作用。为了解ATG10在大豆中的功能，通过菜豆斑驳病毒（BPMV）诱导的基因沉默同时沉默了两个同源基因，即 和 。黑暗处理诱导的碳饥饿以及对ATG8积累水平的蛋白质免疫印迹分析表明，同时沉默 会导致大豆自噬受损；抗病性和激酶分析表明， 通过负调控MPK3/6的激活参与免疫反应，这表明 在大豆免疫反应中起负调控作用。
