从海洋红藻中提取和纯化 R-藻红蛋白α亚基及其生物活性。

Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae and Its Biological Activities.

机构信息

Department of Marine Bio-Materials & Aquaculture, Pukyong National University, Nam-gu, Busan 48513, Korea.

Institute of Fisheries Sciences, Pukyong National University, Gijang-gun, Busan 46041, Korea.

出版信息

Molecules. 2021 Oct 27;26(21):6479. doi: 10.3390/molecules26216479.

Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that is widely used as a fluorescent probe or as a colorant in the food and cosmetic industries. In this study, phycoerythrin was extracted from the red algae and purified by ammonium sulfate precipitation and various chromatography methods. The purified phycoerythrin was analyzed by UV-visible and fluorescence spectroscopy. The isolated pigment had the typical spectrum of R-phycoerythrin, with a trimmer state with absorbance maxima at 497, 536, and 565 nm. It was further purified and identified by LC-MS/MS and Mascot search. It showed a 100% sequence similarity with the R-phycoerythrin alpha subunit of . The molecular mass was 17.97 kDa. The antioxidant activity of the purified R-phycoerythrin alpha subunit was analyzed. It showed significant antioxidant activity in ABTS and FRAP assays and had significant cytotoxicity against HepG2 cells.

藻红蛋白是红藻和蓝藻的主要光捕获色素，广泛用作荧光探针或食品和化妆品工业中的着色剂。在这项研究中，藻红蛋白从红藻中提取出来，通过硫酸铵沉淀和各种色谱方法进行纯化。纯化的藻红蛋白通过紫外可见和荧光光谱进行分析。分离出的色素具有 R-藻红蛋白的典型光谱，具有三聚物状态，最大吸收峰在 497、536 和 565nm 处。它通过 LC-MS/MS 和 Mascot 搜索进一步进行了纯化和鉴定。它与的 R-藻红蛋白α亚基具有 100%的序列相似性。分子量为 17.97kDa。分析了纯化的 R-藻红蛋白α亚基的抗氧化活性。它在 ABTS 和 FRAP 测定中表现出显著的抗氧化活性，对 HepG2 细胞具有显著的细胞毒性。