Molotkovsky Rodion J, Galimzyanov Timur R, Ermakov Yury A
Laboratory of Bioelectrochemistry, A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 31/4 Leninskiy Prospekt, 119071 Moscow, Russia.
Materials (Basel). 2021 Nov 3;14(21):6623. doi: 10.3390/ma14216623.
Natural and synthetic polycations of different kinds attract substantial attention due to an increasing number of their applications in the biomedical industry and in pharmacology. The key characteristic determining the effectiveness of the majority of these applications is the number of macromolecules adsorbed on the surface of biological cells or their lipid models. Their study is complicated by a possible heterogeneity of polymer layer adsorbed on the membrane. Experimental methods reflecting the structure of the layer include the electrokinetic measurements in liposome suspension and the boundary potential of planar bilayer lipid membranes (BLM) and lipid monolayers with a mixed composition of lipids and the ionic media. In the review, we systematically analyze the methods of experimental registration and theoretical description of the laterally heterogeneous structures in the polymer layer published in the literature and in our previous studies. In particular, we consider a model based on classical theory of the electrical double layer, used to analyze the available data of the electrokinetic measurements in liposome suspension with polylysines of varying molecular mass. This model suggests a few parameters related to the heterogeneity of the polymer layer and allows determining the conditions for its appearance at the membrane surface. A further development of this theoretical approach is discussed.
由于不同种类的天然和合成聚阳离子在生物医学产业和药理学中的应用日益增多，它们受到了广泛关注。决定这些应用中大多数有效性的关键特性是吸附在生物细胞表面或其脂质模型上的大分子数量。聚合物层吸附在膜上可能存在的异质性使对它们的研究变得复杂。反映该层结构的实验方法包括脂质体悬浮液中的电动测量以及具有混合脂质和离子介质组成的平面双层脂质膜（BLM）和脂质单层的边界电位。在这篇综述中，我们系统地分析了文献和我们之前研究中发表的聚合物层横向异质结构的实验记录方法和理论描述。特别是，我们考虑了一个基于经典双电层理论的模型，用于分析不同分子量聚赖氨酸脂质体悬浮液中电动测量的现有数据。该模型提出了一些与聚合物层异质性相关的参数，并允许确定其在膜表面出现的条件。还讨论了这种理论方法的进一步发展。
