Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, RS, 90.035-903, Brazil.
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Braz J Microbiol. 2022 Mar;53(1):499-502. doi: 10.1007/s42770-021-00652-7. Epub 2021 Nov 13.
The reservoirs for NDM-producing Enterobacterales are increasing, not only in hospitals, but also in the environment and in the community, challenging the therapeutic efficacy of carbapenems. We aimed to characterize an isolate of Escherichia coli harboring the bla gene recovered from the bloodstream of a penguin (Spheniscus magellanicus) in Southern Brazil. A total of 74 bacterial isolates recovered from arterial blood samples from dead birds were submitted to species identification and antibiotic susceptibility evaluation. One isolate presented resistance to carbapenems (E. coli 89PenNDM) and proved to harbor the bla gene by multiplex high-resolution melting real-time PCR (PCR-HRM). Conjugation experiments indicated that the bla was transmissible to E. coli J53. Whole genome sequencing (WGS) confirmed the presence of the bla gene in a conjugative plasmid (IncA/C plasmid) in both the E. coli 89PenNDM and its transconjugants. The isolate was classified as ST 156 and many other resistance genes (e.g., sul1, sul,2, strA, floR, tet(A)) were identified, all carried in the same IncA/C2 plasmid. This is the first report of bla-producing E. coli isolated from a penguin in the Brazilian seacoast. The presence of a carbapenemase gene in wildlife animals is of concern as they may become reservoirs of multidrug-resistant bacteria and disseminate them to the environment.
产 NDM 肠杆菌科的储库不仅在医院中增加，而且在环境和社区中也在增加，这对碳青霉烯类药物的治疗效果构成了挑战。我们旨在从巴西南部的一只企鹅（Spheniscus magellanicus）的血液中分离出一株携带 bla 基因的大肠杆菌。从死亡鸟类的动脉血液样本中总共分离出 74 株细菌分离株，进行了种属鉴定和抗生素敏感性评估。一株对碳青霉烯类药物（大肠杆菌 89PenNDM）表现出耐药性，并通过多重高分辨率熔解实时 PCR（PCR-HRM）证实携带 bla 基因。接合实验表明 bla 可转移至大肠杆菌 J53。全基因组测序（WGS）证实 bla 基因存在于大肠杆菌 89PenNDM 及其转座子中的一个可接合质粒（IncA/C 质粒）中。该分离株被分类为 ST156，还鉴定出许多其他耐药基因（例如，sul1、sul2、strA、floR、tet(A)），均携带在相同的 IncA/C2 质粒中。这是首次从巴西沿海的企鹅中分离出产 bla 的大肠杆菌。野生动物中存在碳青霉烯酶基因令人担忧，因为它们可能成为多药耐药菌的储库，并将其传播到环境中。
