Wang Jianghui, Zhang Xiaoxue, Wang Xiaojuan, Li Fadi, Zhang Deyin, Li Xiaolong, Zhang Yukun, Zhao Yuan, Song Qizhi, Zhao Liming, Xu Dan, Cheng Jiangbo, Li Wenxin, Zhou Bubo, Lin Changchun, Wang Weimin
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
The State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu, China.
Anim Biotechnol. 2023 Nov;34(4):1626-1634. doi: 10.1080/10495398.2021.1998093. Epub 2021 Nov 13.
Hu sheep is an excellent short fat-tailed breed in China. Fat deposition in Hu sheep tail affects carcass quality and consumes a lot of energy, leading to an increase in feed cost. The objective of this study was to analyze the effects of polymorphism on tail fat weight in Hu sheep. Partial coding and non-coding sequences of were amplified with PCR and single nucleotide polymorphisms (SNP) of in 1163 Hu sheep were detected using DNA sequencing and KASPar technology. RT-qPCR analysis was performed to test expression in different tissues. The results showed that the expression of was higher in the duodenum, liver, spleen, kidney, and lung than in the heart, muscle, rumen, tail fat, and lymph. A mutation, g.5312 C > T, was detected in ; g.5312 C > T was significantly associated with tail fat weight, relative weight of tail fat (body weight), and relative weight of tail fat (carcass) ( < 0.05). The tail fat weight of the genotype was remarkably higher than that of the and genotypes. Therefore, can be used as a genetic marker for marker-assisted selection of tail fat weight in Hu sheep.
湖羊是我国优良的短脂尾绵羊品种。湖羊尾部脂肪沉积影响胴体品质，消耗大量能量，导致饲料成本增加。本研究旨在分析多态性对湖羊尾脂重量的影响。采用PCR扩增部分编码和非编码序列，运用DNA测序和KASPar技术检测1163只湖羊的单核苷酸多态性（SNP）。通过RT-qPCR分析检测其在不同组织中的表达。结果表明，其在十二指肠、肝脏、脾脏、肾脏和肺中的表达高于心脏、肌肉、瘤胃、尾脂和淋巴。在该基因中检测到一个突变，g.5312 C>T；g.5312 C>T与尾脂重量、尾脂相对重量（体重）和尾脂相对重量（胴体）显著相关（P<0.05）。CC基因型的尾脂重量显著高于CT和TT基因型。因此，该基因可作为湖羊尾脂重量标记辅助选择的遗传标记。
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