College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
The State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Anim Biotechnol. 2022 Feb;33(1):90-97. doi: 10.1080/10495398.2020.1775626. Epub 2020 Jun 4.
The aims of this study were to analyze the effects of and polymorphisms on the body weight of Hu sheep. DNA sequencing and KASPar technologies were used to detect single nucleotide polymorphisms (SNPs) within the and genes of Hu sheep ( = 207). Two synonymous mutations, g.300 G > A and g.645 C > T, were detected in and , respectively. The body weights of sheep were recorded at 80, 100, 120, 140, 160 and 180-days, and associations between these polymorphisms and body weight changes were analyzed. Association analysis demonstrated that the polymorphisms in and significantly associated with body weight ( < 0.05). At the g.300 G > A locus, individuals with the GG genotype had significantly higher body weight than those with the AA genotype, and at the g.645 C > T locus, individuals with the TT genotype had significantly higher body weight than those with the TC genotype. Individuals with both polymorphisms exhibited significantly different growth ( < 0.05). These data suggest that polymorphisms in the and genes can be used as candidate molecular markers for the breeding of desirable growth traits in Hu sheep.
本研究旨在分析 和 多态性对湖羊体重的影响。采用 DNA 测序和 KASPar 技术检测湖羊（ = 207） 基因和 基因内的单核苷酸多态性（SNPs）。在 和 中分别检测到两个同义突变，g.300 G > A 和 g.645 C > T。记录了绵羊在 80、100、120、140、160 和 180 天的体重，并分析了这些多态性与体重变化之间的关联。关联分析表明， 和 中的多态性与体重显著相关（ < 0.05）。在 g.300 G > A 位点，GG 基因型个体的体重显著高于 AA 基因型个体，而在 g.645 C > T 位点，TT 基因型个体的体重显著高于 TC 基因型个体。同时具有这两种多态性的个体表现出显著不同的生长（ < 0.05）。这些数据表明， 基因和 基因中的多态性可以作为湖羊理想生长性状选育的候选分子标记。
