Zhao Yuan, Zhang Deyin, Zhang Xiaoxue, Li Fadi, Xu Dan, Zhao Liming, Li Xiaolong, Zhang Yukun, Wang Jianghui, Yang Xiaobing, Wang Weiming
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China.
The State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.
Gene. 2022 Jun 5;826:146451. doi: 10.1016/j.gene.2022.146451. Epub 2022 Mar 28.
Fat mass and obesity associated (FTO) gene is a famous dominant predictor of obesity, and plays pivotal roles in the regulating fatty acid transport and fat metabolism. In the current study, the quantitative real-time PCR (qRT-PCR), DNA-pooled sequencing and KAspar assay were performed to detect the expression features of FTO and the polymorphisms of FTO associated with the tail fat weight related-traits of Hu sheep. The results indicated that the expression of FTO gene is widely expressed in the tissues tested, and the expression level of FTO in tail fat tissue was evidently higher compared with that in the other tissues. In addition, FTO showed the highest expression level in tail fat tissue at three months. The expression of FTO mRNA was lower in the large-tail fat group compared with that in the small-tail fat group in 6 months old. Subsequently, the polymorphism loci g. 23704451C > A detected in the FTO gene was confirmed to be significantly related to the tail length and the weight of tail fat. These results suggested that the polymorphism might be regarded as novel molecular marker for the breeding of small tail sheep.
脂肪量和肥胖相关（FTO）基因是肥胖的一个著名显性预测因子，在调节脂肪酸转运和脂肪代谢中起关键作用。在本研究中，进行了定量实时PCR（qRT-PCR）、DNA混合测序和KASP检测，以检测FTO的表达特征以及与湖羊尾脂重量相关性状相关的FTO多态性。结果表明，FTO基因在所检测的组织中广泛表达，与其他组织相比，FTO在尾脂组织中的表达水平明显更高。此外，FTO在三个月大时在尾脂组织中的表达水平最高。在6月龄时，大尾脂组中FTO mRNA的表达低于小尾脂组。随后，在FTO基因中检测到的多态性位点g.23704451C>A被证实与尾长和尾脂重量显著相关。这些结果表明，该多态性可能被视为小尾羊育种的新型分子标记。
