College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
The State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu, China.
Anim Biotechnol. 2023 Dec;34(8):3287-3293. doi: 10.1080/10495398.2022.2138413. Epub 2022 Nov 8.
Growth traits are important economic characteristics of livestock and poultry. In the present study, the expression features of and the relationship between gene polymorphisms and growth traits in Hu sheep were investigated by using real-time quantitative PCR technology (qPCR), Sanger sequencing, and Kaspar genotyping technology. The qPCR results showed that the gene is expressed widely in the tested tissues of Hu sheep, and the expression level of the gene in the heart and the muscle was significantly higher than in other tissues ( < 0.05). Missense mutation c.62565119 A > G was found in , and an association analysis showed that it was correlated with the growth traits (body weight, body height, and body length) of Hu sheep ( < 0.05). The body weight, body height, and body length of the sheep carrying the AA genotype were remarkably higher than those of the GG and AG genotypes ( < 0.05). These results showed that novel polymorphisms at the gene can be used as a genetic marker of growth traits of Hu sheep.
生长性状是家畜家禽重要的经济特征。本研究采用实时荧光定量 PCR（qPCR）技术、Sanger 测序和 Kompas 基因分型技术，研究了 Hu 绵羊中基因的表达特征及其基因多态性与生长性状的关系。qPCR 结果表明，基因在 Hu 绵羊检测的组织中广泛表达，且基因在心和肌肉中的表达水平显著高于其他组织（<0.05）。在中发现了错义突变 c.62565119 A>G，关联分析表明它与 Hu 绵羊的生长性状（体重、体高和体长）相关（<0.05）。携带 AA 基因型的绵羊的体重、体高和体长显著高于 GG 和 AG 基因型（<0.05）。这些结果表明，基因的新多态性可以作为 Hu 绵羊生长性状的遗传标记。
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