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青梅果实浓缩提取物通过抑制脂肪生成和诱导米色化/褐色化，对3T3-L1脂肪细胞发挥双重作用。

Concentrated extract of Prunus mume fruit exerts dual effects in 3T3-L1 adipocytes by inhibiting adipogenesis and inducing beiging/browning.

作者信息

Bu Su, Yuan Chunying, Cao Fuliang, Xu Qifeng, Zhang Yichun, Ju Ronghua, Chen Longyun, Li Zhong

机构信息

College of Biology and the Environment, Nanjing Forestry University, Nanjing, China.

These authors contributed equally to this study.

出版信息

Food Nutr Res. 2021 Oct 29;65. doi: 10.29219/fnr.v65.5492. eCollection 2021.

DOI:10.29219/fnr.v65.5492
PMID:34776833
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8559450/
Abstract

BACKGROUND

The fruit has beneficial effects in the treatment of obesity and metabolic syndrome. However, its mechanism of action is unclear.

OBJECTIVE

We assessed the effect of a concentrated water extract of fruit (CEPM) on adipogenesis and beiging/browning in 3T3-L1 cells.

METHODS

The cell viability was determined by MTT assay. Lipid accumulation was assessed with Oil Red O (ORO) staining under different concentrations of CEPM. The effects of CEPM treatment during differentiation on beiging/browning and mitochondrial biogenesis in 3T3-L1 cells were investigated.

RESULTS

CEPM treatment suppressed differentiation and decreased lipid accumulation by downregulating the expression of key adipogenic genes, including PPARγ, C/EBPα, SREBP-1c, FAS, and perilipin A. In contrast, CEPM treatment increased the mitochondrial DNA (mtDNA) content and mRNA levels of mitochondrial biogenesis genes, including , , and α, and reduced reactive oxygen species levels. Importantly, CEPM increased the expression of brown/beige hallmark genes (α, , , , , , and ), as well as proteins (UCP1, PGC-1α, NRF1, TBX1, and CPT1α). The high-performance liquid chromatography (HPLC) analysis reveals that CEPM contains mumefural, naringin, 5-HMF, citric acid, caffeic acid, and hesperidin.

CONCLUSION

The first evidence we provided showed that CEPM has a dual role in 3T3-L1 cells inhibiting adipogenesis and promoting beiging/browning, and hence, could be a potential agent in the fight against obesity.

摘要

背景

该水果在肥胖症和代谢综合征的治疗中具有有益作用。然而，其作用机制尚不清楚。

目的

我们评估了该水果浓缩水提取物（CEPM）对3T3-L1细胞脂肪生成和米色化/褐色化的影响。

方法

通过MTT法测定细胞活力。在不同浓度的CEPM作用下，用油红O（ORO）染色评估脂质积累情况。研究了CEPM在分化过程中对3T3-L1细胞米色化/褐色化和线粒体生物发生的影响。

结果

CEPM处理通过下调关键脂肪生成基因（包括PPARγ、C/EBPα、SREBP-1c、FAS和 perilipin A）的表达来抑制分化并减少脂质积累。相比之下，CEPM处理增加了线粒体DNA（mtDNA）含量以及线粒体生物发生基因（包括 、 、 和α）的mRNA水平，并降低了活性氧水平。重要的是，CEPM增加了棕色/米色标志性基因（α、 、 、 、 、 和 ）以及蛋白质（UCP1、PGC-1α、NRF1、TBX1和CPT1α）的表达。高效液相色谱（HPLC）分析表明，CEPM含有木犀草素、柚皮苷、5-羟甲基糠醛、柠檬酸、咖啡酸和橙皮苷。

结论

我们提供的首个证据表明，CEPM在3T3-L1细胞中具有抑制脂肪生成和促进米色化/褐色化的双重作用，因此，可能是对抗肥胖的潜在药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/041968160469/FNR-65-5492-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/e2fed9a10612/FNR-65-5492-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/9a38cc97787a/FNR-65-5492-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/0dbcd5b3215d/FNR-65-5492-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/68e3540ec065/FNR-65-5492-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/7035bb4b0df0/FNR-65-5492-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/750d356dec59/FNR-65-5492-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/041968160469/FNR-65-5492-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/e2fed9a10612/FNR-65-5492-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/9a38cc97787a/FNR-65-5492-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/0dbcd5b3215d/FNR-65-5492-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/68e3540ec065/FNR-65-5492-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/7035bb4b0df0/FNR-65-5492-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/750d356dec59/FNR-65-5492-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8559450/041968160469/FNR-65-5492-g007.jpg

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