Hamza Rim, Roque Edelín, Gómez-Mena Concepción, Madueño Francisco, Beltrán José Pío, Cañas Luis A
Department of Plant Development and Hormone Action, Instituto de Biología Molecular y Celular de Plantas (CSIC-Universitat Politècnica de València), Ciudad Politécnica de la Innovación, Valencia, Spain.
Front Plant Sci. 2021 Oct 29;12:765277. doi: 10.3389/fpls.2021.765277. eCollection 2021.
Redox homeostasis has been linked to proper anther and pollen development. Accordingly, plant cells have developed several Reactive Oxygen Species (ROS)-scavenging mechanisms to maintain the redox balance. Hemopexins constitute one of these mechanisms preventing heme-associated oxidative stress in animals, fungi, and plants. ) is a pea anther-specific gene that encodes a protein containing four hemopexin domains. We report the functional characterization of and the identification in its promoter region of -regulatory elements that are essential for the specific expression in anthers. promoter deletion analysis revealed that a putative CArG-like regulatory motif is necessary to confer promoter activity in developing anthers. Our data suggest that might be a hemopexin regulated by a MADS-box protein. gene silencing in pea, and its overexpression in heterologous systems, result in similar defects in the anthers consisting of precocious tapetum degradation and the impairment of pollen development. Such alterations were associated to the production of superoxide anion and altered activity of ROS-scavenging enzymes. Our findings demonstrate that is essential for pollen development by modulating ROS levels during the differentiation of the anther tissues surrounding the microsporocytes.
氧化还原稳态与花药和花粉的正常发育有关。因此，植物细胞已经发展出几种活性氧（ROS）清除机制来维持氧化还原平衡。血红素结合蛋白构成了其中一种机制，可防止动物、真菌和植物中与血红素相关的氧化应激。）是一个豌豆花药特异性基因，编码一种含有四个血红素结合蛋白结构域的蛋白质。我们报告了该基因的功能特征，并在其启动子区域鉴定了对花药特异性表达至关重要的调控元件。启动子缺失分析表明，一个假定的类CArG调控基序对于赋予发育中的花药启动子活性是必要的。我们的数据表明，该基因可能是一个受MADS盒蛋白调控的血红素结合蛋白。豌豆中该基因的沉默及其在异源系统中的过表达，导致花药出现类似缺陷，包括绒毡层过早降解和花粉发育受损。这些改变与超氧阴离子的产生以及ROS清除酶活性的改变有关。我们的研究结果表明，该基因通过在小孢子母细胞周围的花药组织分化过程中调节ROS水平，对花粉发育至关重要。
