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A cis-acting element is necessary and sufficient for translational regulation of human ferritin expression in response to iron.

作者信息

Hentze M W, Rouault T A, Caughman S W, Dancis A, Harford J B, Klausner R D

机构信息

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.

出版信息

Proc Natl Acad Sci U S A. 1987 Oct;84(19):6730-4. doi: 10.1073/pnas.84.19.6730.

DOI:10.1073/pnas.84.19.6730
PMID:3477805
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC299157/
Abstract

Ferritin plays a key role in determining the intracellular fate of iron and is highly regulated by the iron status of the cell. We have identified a cis-acting element in the transcribed but nontranslated 5' leader sequence of human ferritin heavy-chain mRNA. In transiently transfected murine fibroblasts, the presence of a 157-nucleotide region of the 5' leader sequence was found to be necessary for iron-dependent regulation of ferritin biosynthesis. Further, this 5' leader region is sufficient to transfer iron-mediated translational control to the expression of a heterologous gene product, chloramphenicol acetyltransferase.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/ea50784222c0/pnas00334-0130-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/579252f87187/pnas00334-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/91860065f78e/pnas00334-0129-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/50c85f5eb9cb/pnas00334-0129-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/5d308714d03c/pnas00334-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/591c21b0330b/pnas00334-0130-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/0b878aa37550/pnas00334-0130-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/ea50784222c0/pnas00334-0130-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/579252f87187/pnas00334-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/91860065f78e/pnas00334-0129-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/50c85f5eb9cb/pnas00334-0129-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/5d308714d03c/pnas00334-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/591c21b0330b/pnas00334-0130-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/0b878aa37550/pnas00334-0130-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8abf/299157/ea50784222c0/pnas00334-0130-d.jpg

相似文献

1
A cis-acting element is necessary and sufficient for translational regulation of human ferritin expression in response to iron.
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6730-4. doi: 10.1073/pnas.84.19.6730.
2
Identification of the iron-responsive element for the translational regulation of human ferritin mRNA.人铁蛋白mRNA翻译调控中铁反应元件的鉴定
Science. 1987 Dec 11;238(4833):1570-3. doi: 10.1126/science.3685996.
3
Iron-responsive elements: regulatory RNA sequences that control mRNA levels and translation.铁反应元件：控制mRNA水平和翻译的调控RNA序列。
Science. 1988 May 13;240(4854):924-8. doi: 10.1126/science.2452485.
4
The iron-responsive element is the single element responsible for iron-dependent translational regulation of ferritin biosynthesis. Evidence for function as the binding site for a translational repressor.铁反应元件是负责铁蛋白生物合成的铁依赖性翻译调控的单一元件。有证据表明其作为翻译阻遏物结合位点的功能。
J Biol Chem. 1988 Dec 15;263(35):19048-52.
5
The influence of the base-paired flanking region on structure and function of the ferritin mRNA iron regulatory element.碱基配对侧翼区域对铁蛋白mRNA铁调节元件的结构和功能的影响。
J Mol Biol. 1993 May 20;231(2):230-40. doi: 10.1006/jmbi.1993.1278.
6
Role of RNA secondary structure of the iron-responsive element in translational regulation of ferritin synthesis.铁反应元件的RNA二级结构在铁蛋白合成翻译调控中的作用。
Nucleic Acids Res. 1995 Oct 25;23(20):4190-5. doi: 10.1093/nar/23.20.4190.
7
Ferritin translation by interleukin-1and interleukin-6: the role of sequences upstream of the start codons of the heavy and light subunit genes.白细胞介素-1和白细胞介素-6对铁蛋白的翻译作用：重链和轻链亚基基因起始密码子上游序列的作用
Blood. 1996 Mar 15;87(6):2525-37.
8
Translational enhancement of H-ferritin mRNA by interleukin-1 beta acts through 5' leader sequences distinct from the iron responsive element.白细胞介素-1β对H-铁蛋白mRNA的翻译增强作用通过不同于铁反应元件的5'前导序列发挥作用。
Nucleic Acids Res. 1994 Jul 11;22(13):2678-86. doi: 10.1093/nar/22.13.2678.
9
Induction of ferritin subunit synthesis by iron is regulated at both the transcriptional and translational levels.铁对铁蛋白亚基合成的诱导在转录和翻译水平上均受到调控。
J Biol Chem. 1988 Jun 25;263(18):8938-42.
10
Iron regulation of ferritin gene expression.铁对铁蛋白基因表达的调控。
J Cell Biochem. 1990 Oct;44(2):107-15. doi: 10.1002/jcb.240440205.

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本文引用的文献

1
Iron transport and storage proteins.铁转运与储存蛋白。
Annu Rev Biochem. 1980;49:357-93. doi: 10.1146/annurev.bi.49.070180.002041.
2
Translational control of ferritin synthesis by iron in embryonic reticulocytes of the bullfrog.牛蛙胚胎网织红细胞中铁对铁蛋白合成的翻译调控。
J Biol Chem. 1982 Dec 10;257(23):14187-91.
3
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
弓形虫中铁介导的转录后调控
PLoS Pathog. 2025 Feb 3;21(2):e1012857. doi: 10.1371/journal.ppat.1012857. eCollection 2025 Feb.
4
Host-like RNA Elements Regulate Virus Translation.宿主样 RNA 元件调节病毒翻译。
Viruses. 2024 Mar 20;16(3):468. doi: 10.3390/v16030468.
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Single-molecule imaging reveals translation-dependent destabilization of mRNAs.单分子成像揭示了翻译依赖性的 mRNA 不稳定性。
Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
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Translation of SARS-CoV-2 gRNA Is Extremely Efficient and Competitive despite a High Degree of Secondary Structures and the Presence of an uORF.SARS-CoV-2 gRNA 翻译效率极高且具有竞争性，尽管其具有高度的二级结构和 uORF 的存在。
Viruses. 2022 Jul 8;14(7):1505. doi: 10.3390/v14071505.
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Orchestrating a heist: uptake and storage of metals by apicomplexan parasites.精心策划的抢劫：顶复门寄生虫对金属的摄取和储存。
Microbiology (Reading). 2021 Dec;167(12). doi: 10.1099/mic.0.001114.
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Knockout fth1b affects early mineralization of zebrafish pharyngeal teeth.敲除 fth1b 会影响斑马鱼咽齿的早期矿化。
Hua Xi Kou Qiang Yi Xue Za Zhi. 2021 Feb 1;39(1):32-37. doi: 10.7518/hxkq.2021.01.005.
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Iron Regulatory Protein 2 Exerts its Oncogenic Activities by Suppressing TAp63 Expression.铁调节蛋白2通过抑制TAp63的表达发挥其致癌活性。
Mol Cancer Res. 2020 Jul;18(7):1039-1049. doi: 10.1158/1541-7786.MCR-19-1104. Epub 2020 Apr 10.
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4
Regulation of ferritin mRNA: a possible gene-sparing phenomenon. Induction of ferritin synthesis by iron in liver as well as red cells combines high translational efficiency with increased utilization of preformed ferritin mRNA.铁蛋白mRNA的调控：一种可能的基因节省现象。铁在肝脏以及红细胞中诱导铁蛋白合成，这一过程将高翻译效率与对预先形成的铁蛋白mRNA的利用率增加相结合。
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5
Isolation and characterization of a cDNA clone for human ferritin heavy chain.人铁蛋白重链cDNA克隆的分离与鉴定
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6
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7
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Cell. 1985 Oct;42(3):903-11. doi: 10.1016/0092-8674(85)90286-7.