Costanzo F, Santoro C, Colantuoni V, Bensi G, Raugei G, Romano V, Cortese R
EMBO J. 1984 Jan;3(1):23-7. doi: 10.1002/j.1460-2075.1984.tb01756.x.
We have cloned a segment of cDNA from human liver coding for an apoferritin subunit, probably an H chain. Sequence comparison with the available protein sequence shows that our clone corresponds to a ferritin subunit present as a minor species in human spleen and placenta, but as major species in HeLa cells. Northern blot analysis shows the existence of only one band of similar size in human liver, HeLa cells, Daudi lymphoma and Hep3B hepatoma cell lines. In contrast, Southern blot analysis provides evidence for a multigene family.
我们从人肝脏中克隆了一段编码脱铁铁蛋白亚基（可能是H链）的cDNA片段。与现有蛋白质序列的序列比较表明，我们的克隆对应于一种在人脾脏和胎盘中以次要形式存在，但在HeLa细胞中以主要形式存在的铁蛋白亚基。Northern印迹分析表明，在人肝脏、HeLa细胞、Daudi淋巴瘤和Hep3B肝癌细胞系中仅存在一条大小相似的条带。相比之下，Southern印迹分析为一个多基因家族提供了证据。
