Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Graduate School, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Front Cell Infect Microbiol. 2021 Oct 29;11:739496. doi: 10.3389/fcimb.2021.739496. eCollection 2021.
() is an ascomycete yeast species widely used in environmental and industrial research and capable of causing infections in humans and animals. At present, there are only a few studies on , and further research is required for its more in-depth characterization and analysis. Eleven strains of collected from China Hospital Invasive Fungal Surveillance Net (CHIF-NET) and the CHIF-NET North China Program were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry and internal transcribed spacer sequencing. The antifungal susceptibility of the strains was tested using the Clinical and Laboratory Standards Institute broth microdilution method and Sensititre YeastOne™. Furthermore, ERG11 and FKS1 were sequenced to determine any mutations related to azole and echinocandin resistance in . All isolates exhibited low minimum inhibitory concentration (MIC) values for itraconazole (0.06-0.12 μg/ml), posaconazole (0.06-0.12 μg/ml), amphotericin B (0.25-1 μg/ml), and 5-flucytosine (range, <0.06-0.12 μg/ml), whereas four isolates showed high MICs (≥4 μg/ml) for echinocandins. Strains with high MIC values for azoles showed common mutations, namely, F126L/K143R. In addition, L139R mutations may be linked to high MICs of fluconazole. Two amino acid alterations reported to correspond to high MIC values of echinocandin, namely, F621I (F641) and S625L (S645), were found in the hot spot 1 region of . In addition, one new amino acid alteration, I1348S (I1368), was found outside of the hot spot 2 region, and its contribution to echinocandin resistance requires future investigation. mainly infects patients with a weak immune system, and the high MIC values for various antifungals exhibited by these isolates may represent a challenge to clinical treatment.
() 是一种子囊菌酵母物种，广泛应用于环境和工业研究，并能引起人类和动物的感染。目前，关于 的研究较少，需要进一步研究以更深入地描述和分析它。从中国医院侵袭性真菌监测网 (CHIF-NET) 和 CHIF-NET 华北项目中收集的 11 株 采用基质辅助激光解吸电离飞行时间质谱和内部转录间隔区测序进行鉴定。采用临床和实验室标准协会肉汤微量稀释法和 Sensititre YeastOne™ 测定 菌株的抗真菌药敏性。此外，还对 ERG11 和 FKS1 进行测序，以确定 中与唑类和棘白菌素耐药相关的任何突变。所有分离株对伊曲康唑 (0.06-0.12 μg/ml)、泊沙康唑 (0.06-0.12 μg/ml)、两性霉素 B (0.25-1 μg/ml) 和 5-氟胞嘧啶 (范围，<0.06-0.12 μg/ml) 的最低抑菌浓度 (MIC) 值均较低，而 4 株分离株对棘白菌素的 MIC 值较高 (≥4 μg/ml)。唑类药物 MIC 值较高的菌株表现出常见的 F126L/K143R 突变。此外，L139R 突变可能与氟康唑的高 MIC 值有关。在 热点 1 区域发现了与棘白菌素高 MIC 值相关的两种氨基酸改变，即 F621I (F641) 和 S625L (S645)。此外，还发现了一个新的氨基酸改变，I1348S (I1368)，位于 热点 2 区域之外，其对棘白菌素耐药的贡献需要进一步研究。 主要感染免疫系统较弱的患者，这些分离株对各种抗真菌药物的高 MIC 值可能对临床治疗构成挑战。
